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MB Sample ID: SA003775
Local Sample ID: | 6653 |
Subject ID: | SU000092 |
Subject Type: | Animal |
Subject Species: | Macaca fascicularis |
Taxonomy ID: | 9541 |
Age Or Age Range: | 7.5-18.17 |
Gender: | Female |
Animal Housing: | pen |
Animal Feed: | Prudent #611 or Wester #907 |
Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000092 |
Subject Type: | Animal |
Subject Species: | Macaca fascicularis |
Taxonomy ID: | 9541 |
Age Or Age Range: | 7.5-18.17 |
Gender: | Female |
Animal Housing: | pen |
Animal Feed: | Prudent #611 or Wester #907 |
Species Group: | Mammal |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
6653 | SA003775 | FL000962 | Prudent_#611 | Diet |
6653 | SA003775 | FL000962 | 13.17 | Age |
Collection:
Collection ID: | CO000075 |
Collection Summary: | - |
Collection Protocol Comments: | Snap frozen in liquid nitrogen |
Sample Type: | Ovary tissue |
Volumeoramount Collected: | 128-597mg |
Storage Conditions: | -80C |
Treatment:
Treatment ID: | TR000093 |
Sample Preparation:
Sampleprep ID: | SP000088 |
Sampleprep Summary: | Ovary samples were extracted using acetonitrile protein precipitation. Samples were then dried and reconstituted for LC-MS analysis in 95:5 water:methanol. |
Sampleprep Protocol Filename: | RTI_APPT-SERUM_Metabolomics_Procedure.docx |
Processing Method: | Homogenization |
Extraction Method: | Acetonitrile |
Extract Storage: | -80 C |
Sample Resuspension: | 95:5 Water:Methanol |
Sample Spiking: | L-Tryptophan-d5 |
Organ: | Ovary |
Combined analysis:
Analysis ID | AN000115 | AN000116 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Waters Acquity | Waters Acquity |
Column | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) |
MS Type | ESI | ESI |
MS instrument type | QTOF | QTOF |
MS instrument name | Waters Synapt G2 | Waters Synapt G2 |
Ion Mode | POSITIVE | NEGATIVE |
Units | Normalized abundance | Normalized abundance |
Chromatography:
Chromatography ID: | CH000079 |
Instrument Name: | Waters Acquity |
Column Name: | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) |
Column Pressure: | 6000-10000 |
Column Temperature: | 50 C |
Flow Gradient: | Time(min) Flow Rate %A %B Curve ; 1. Initial 0.400 100.0 0.0 ; 2. 1.00 0.400 100.0 0.0 6 ; 3. 16.00 0.400 0.0 100.0 6 ; 4. 20.00 0.400 0.0 100.0 6 ; 5. 22.00 0.400 100.0 0.0 6 |
Flow Rate: | 0.4 mL/min |
Injection Temperature: | 8 C |
Internal Standard: | L-Tryptophan-d5 |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% methanol; 0.1% formic acid |
Analytical Time: | 22 min |
Weak Wash Solvent Name: | 5%MeOH |
Weak Wash Volume: | 1000 uL |
Strong Wash Solvent Name: | 80%MeOH |
Strong Wash Volume: | 1000 uL |
Target Sample Temperature: | 8 C |
Sample Loop Size: | 10 uL |
Sample Syringe Size: | 100 uL |
Randomization Order: | Yes |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS000091 |
Analysis ID: | AN000115 |
Instrument Name: | Waters Synapt G2 |
Instrument Type: | QTOF |
MS Type: | ESI |
Ion Mode: | POSITIVE |
Capillary Temperature: | 110 C |
Capillary Voltage: | 3.2 kV |
Collision Energy: | 4 |
Fragmentation Method: | CID |
Helium Flow: | 180 |
Ionization: | ES+ |
Mass Accuracy: | 10 ppm |
Source Temperature: | 110 C |
Dataformat: | Continuum |
Desolvation Gas Flow: | 400 L/Hr |
Desolvation Temperature: | 400 C |
Resolution Setting: | 18000 |
Scan Range Moverz: | 50-1000 m/s |
Scanning Cycle: | 1 s |
Tube Lens Voltage: | 75 |
MS ID: | MS000092 |
Analysis ID: | AN000116 |
Instrument Name: | Waters Synapt G2 |
Instrument Type: | QTOF |
MS Type: | ESI |
Ion Mode: | NEGATIVE |
Capillary Temperature: | 110 C |
Capillary Voltage: | 2.8 kV |
Collision Energy: | 4 |
Fragmentation Method: | CID |
Helium Flow: | 180 |
Ionization: | ES- |
Source Temperature: | 110 C |
Dataformat: | Continuum |
Desolvation Gas Flow: | 400 L/Hr |
Desolvation Temperature: | 400 C |
Resolution Setting: | 18000 |
Scan Range Moverz: | 50-1000 m/z |
Scanning Cycle: | 1 s |
Tube Lens Voltage: | 74 |