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MB Sample ID: SA005266
Local Sample ID: | Patient_141_FFA_A |
Subject ID: | SU000112 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Age Or Age Range: | postmenopausal women |
Gender: | Female |
Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000112 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Age Or Age Range: | postmenopausal women |
Gender: | Female |
Species Group: | Human |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
Patient_141_FFA_A | SA005266 | FL001341 | FFA affected | Condition |
Patient_141_FFA_A | SA005266 | FL001341 | frontal scalp | location |
Collection:
Collection ID: | CO000095 |
Collection Summary: | - |
Sample Type: | 4 mm Scalp biopsies |
Treatment:
Treatment ID: | TR000113 |
Treatment Summary: | No treatment |
Sample Preparation:
Sampleprep ID: | SP000108 |
Sampleprep Summary: | Metabolons standard solvent extraction method. The sample preparation process was carried out using the automated MicroLab STAR® system from Hamilton Company. Recovery standards were added prior to the first step in the extraction process for QC purposes. Sample preparation was conducted using a proprietary series of organic and aqueous extractions to remove the protein fraction while allowing maximum recovery of small molecules. The resulting extract was divided into two fractions; one for analysis by LC and one for analysis by GC. Samples were placed briefly on a TurboVap® (Zymark) to remove the organic solvent. Each sample was then frozen and dried under vacuum. Samples were then prepared for the appropriate instrument, either LC/MS or GC/MS. |
Sampleprep Protocol Filename: | Metabolon_Methods_CASE-03-11VW.docx |
Sample Derivatization: | 50?L for GC/MS analysis using equal parts bistrimethyl-silyl-trifluoroacetamide and solvent mixture |
Combined analysis:
Analysis ID | AN000147 | AN000148 | AN000149 |
---|---|---|---|
Analysis type | MS | MS | MS |
Chromatography type | GC | GC | GC |
Chromatography system | |||
Column | GCMS-5% phenyldimethyl silicone,2.1 mm 100 mm Waters BEH C18 1.7um particles | GCMS-5% phenyldimethyl silicone,2.1 mm 100 mm Waters BEH C18 1.7um particles | GCMS-5% phenyldimethyl silicone,2.1 mm 100 mm Waters BEH C18 1.7um particles |
MS Type | EI | ESI | ESI |
MS instrument type | Single quadrupole | Single quadrupole | Single quadrupole |
MS instrument name | Thermo Trace DSQ | Thermo LTQ | Thermo LTQ |
Ion Mode | POSITIVE | POSITIVE | NEGATIVE |
Units | Unspecified | Unspecified | Unspecified |
Chromatography:
Chromatography ID: | CH000105 |
Chromatography Summary: | GC/MS and LC/MS/MS platforms |
Chromatography Comments: | HPLC-3 ?m particle 2.1 mm 100 mm Aquasil C18 (ThermoFisher) column.LTQ mass spectrometer (Thermo Fisher Scientific, Inc., Waltham, MA),Thermo-Finnigan Trace DSQ MS (Thermo Fisher Scientific, Inc.) |
Column Name: | GCMS-5% phenyldimethyl silicone,2.1 mm 100 mm Waters BEH C18 1.7um particles |
Column Temperature: | 40 C |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% methanol; 0.1% formic acid |
Chromatography Type: | GC |
MS:
MS ID: | MS000123 |
Analysis ID: | AN000147 |
Instrument Name: | Thermo Trace DSQ |
Instrument Type: | Single quadrupole |
MS Type: | EI |
MS Comments: | For GC/MS analysis, samples were analyzed on a Thermo-Finnigan Trace DSQ fast-scanning single-quadrupole mass spectrometer using electron impact ionization. |
Ion Mode: | POSITIVE |
Capillary Temperature: | 60°C to 340°C |
MS ID: | MS000124 |
Analysis ID: | AN000148 |
Instrument Name: | Thermo LTQ |
Instrument Type: | Single quadrupole |
MS Type: | ESI |
MS Comments: | The LC/MS portion of the platform was based on a Waters ACQUITY UPLC and a Thermo-Finnigan LTQ mass spectrometer, which consisted of an electrospray ionization (ESI) source and linear ion-trap (LIT) mass analyzer. |
Ion Mode: | POSITIVE |
Capillary Temperature: | 60°C to 340°C |
MS ID: | MS000125 |
Analysis ID: | AN000149 |
Instrument Name: | Thermo LTQ |
Instrument Type: | Single quadrupole |
MS Type: | ESI |
MS Comments: | The LC/MS portion of the platform was based on a Waters ACQUITY UPLC and a Thermo-Finnigan LTQ mass spectrometer, which consisted of an electrospray ionization (ESI) source and linear ion-trap (LIT) mass analyzer. |
Ion Mode: | NEGATIVE |
Capillary Temperature: | 60°C to 340°C |