Return to study ST000097 main page
MB Sample ID: SA005462
| Local Sample ID: | H2_19 |
| Subject ID: | SU000116 |
| Subject Type: | Insect |
| Subject Species: | Drosophila melanogaster |
| Taxonomy ID: | 7227 |
| Gender: | Female |
| Species Group: | Insect |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000116 |
| Subject Type: | Insect |
| Subject Species: | Drosophila melanogaster |
| Taxonomy ID: | 7227 |
| Gender: | Female |
| Species Group: | Insect |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| H2_19 | SA005462 | FL001372 | Cold Hardy | Cold Susceptibility |
Collection:
| Collection ID: | CO000099 |
| Collection Summary: | Polar-phase metabolites were extracted from homogenized whole fly tissue using an aqueous-optimized metabolite extraction protocol. (Wu, H.; Southam, A. D.; Hines, A.; Viant, M. R. Analytical biochemistry 2008.) |
| Sample Type: | Tissue extract |
Treatment:
| Treatment ID: | TR000117 |
Sample Preparation:
| Sampleprep ID: | SP000112 |
| Sampleprep Summary: | Twenty replicates of each genetic line (cold hardy and cold tolerant) were collected with 40 flies per replicate. Polar phase extracted metabolites were dried and reconstituted in 40 ?L of 99% D2O and pipetted into 1.5-mm NMR tubes. |
| Processing Method: | Lyophilization |
| Processing Storage Conditions: | -80 degree celsius |
| Extraction Method: | Polar phase extraction |
Analysis:
| MB Sample ID: | SA005462 |
| Analysis ID: | AN000155 |
| Laboratory Name: | Edison |
| Analysis Type: | NMR |
| Analysis Comments: | 1D 13C |
| Software Version: | VNMRJ |
| Operator Name: | Chaevien Clendinen |
| Data Format: | Varian |
| Chromatography ID: | CH000111 |
| Num Factors: | 2 |
| MB Sample ID: | SA005462 |
| Analysis ID: | AN000156 |
| Laboratory Name: | Edison |
| Analysis Type: | NMR |
| Analysis Comments: | 1D 1H |
| Software Version: | VNMRJ |
| Operator Name: | Chaevien Clendinen |
| Data Format: | Varian |
| Chromatography ID: | CH000111 |
| Num Factors: | 2 |
NMR:
| NMR ID: | NM000027 |
| Analysis ID: | AN000155 |
| Instrument Name: | Varian |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D 13C |
| Spectrometer Frequency: | 600 MHz |
| NMR Probe: | 1.5 mm HTS cryoprobe |
| NMR Solvent: | D2O |
| NMR Tube Size: | 1.5 mm |
| Shimming Method: | Manual |
| Pulse Sequence: | 1D 13C |
| Pulse Width: | 60 |
| Receiver Gain: | 28 |
| Temperature: | 22 degree Celsius |
| Number Of Scans: | 8192 |
| Dummy Scans: | 16 |
| Acquisition Time: | 0.8s |
| Relaxation Delay: | 0.1 |
| Spectral Width: | 212 ppm |
| Num Data Points Acquired: | 51282 |
| Real Data Points: | 25641 |
| Line Broadening: | 2 Hz |
| Zero Filling: | 2x |
| Apodization: | Exponential |
| NMR ID: | NM000028 |
| Analysis ID: | AN000156 |
| Instrument Name: | Varian |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D 1H |
| Spectrometer Frequency: | 600 MHz |
| NMR Probe: | 1.5 mm HTS cryoprobe |
| NMR Solvent: | D2O |
| NMR Tube Size: | 1.5 mm |
| Shimming Method: | Manual |
| Pulse Sequence: | 1D 1H |
| Water Suppression: | Presaturation |
| Pulse Width: | 45 |
| Receiver Gain: | 28 |
| Offset Frequency: | 4.77 |
| Chemical Shift Ref Cpd: | TSP |
| Temperature: | 22 degree Celsius |
| Number Of Scans: | 64 |
| Dummy Scans: | 16 |
| Acquisition Time: | 2.3s |
| Relaxation Delay: | 2s |
| Spectral Width: | 12 ppm |
| Num Data Points Acquired: | 32768 |
| Real Data Points: | 16384 |
| Zero Filling: | 2x |
| Apodization: | Cosine squared |
