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MB Sample ID: SA005626
Local Sample ID: | 318 |
Subject ID: | SU000121 |
Subject Type: | Insect |
Subject Species: | Drosophila melanogaster |
Taxonomy ID: | 7227 |
Genotype Strain: | Originating from same base population as Drosophila melanogaster genetic reference panel, selected for fast (hardy) and slow (susceptible) recovery from chill coma. |
Age Or Age Range: | 5-7 day-old adults (mated) |
Weight Or Weight Range: | 0.17-0.77mg |
Height Or Height Range: | NA |
Gender: | Female |
Animal Housing: | 25 degC |
Animal Light Cycle: | 12L:12D |
Animal Feed: | Standard cornmeal molasses medium |
Animal Water: | in diet only |
Species Group: | Insect |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000121 |
Subject Type: | Insect |
Subject Species: | Drosophila melanogaster |
Taxonomy ID: | 7227 |
Genotype Strain: | Originating from same base population as Drosophila melanogaster genetic reference panel, selected for fast (hardy) and slow (susceptible) recovery from chill coma. |
Age Or Age Range: | 5-7 day-old adults (mated) |
Weight Or Weight Range: | 0.17-0.77mg |
Height Or Height Range: | NA |
Gender: | Female |
Animal Housing: | 25 degC |
Animal Light Cycle: | 12L:12D |
Animal Feed: | Standard cornmeal molasses medium |
Animal Water: | in diet only |
Species Group: | Insect |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
318 | SA005626 | FL001434 | before | time |
318 | SA005626 | FL001434 | susceptible | hardiness |
318 | SA005626 | FL001434 | 1 | population replicate |
Collection:
Collection ID: | CO000104 |
Collection Summary: | Flies are snap-frozen in liquid nitrogen, counted into pools of 10, and stored at -80 degC until extraction. |
Collection Protocol Filename: | Fly_rearing_and_sample_collection_24Sept2014.docx |
Sample Type: | whole bodies |
Collection Method: | Snap-freeze in liquid nitrogen |
Collection Location: | University of Florida |
Storage Conditions: | -80 degC |
Collection Vials: | 2mL eppendorf tubes |
Storage Vials: | 2mL eppendorf tubes |
Treatment:
Treatment ID: | TR000122 |
Treatment Summary: | Flies are exposed to 3h in an ice slurry, then allowed to recover at room temperature for 5min. |
Treatment Protocol Filename: | Fly_rearing_and_sample_collection_24Sept2014.docx |
Treatment: | abiotic - cold exposure, 3h in ice slurry |
Treatment Dose: | ice slurry |
Treatment Doseduration: | 3h |
Animal Endp Tissue Coll List: | 10 whole flies/sample |
Sample Preparation:
Sampleprep ID: | SP000117 |
Sampleprep Summary: | - |
Sampleprep Protocol Filename: | sample_prep_protocol_24Sept2014.docx sample_spiking_protocol_24Sept2014.docx |
Sampleprep Protocol Comments: | Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37: 911-7. |
Processing Method: | lyophilization, homogenization, solvent extraction |
Processing Storage Conditions: | store at -20C |
Extraction Method: | modified Bligh and Dyer protocol |
Extract Concentration Dilution: | 2:2:1.8 (methanol:chloroform:water) |
Extract Storage: | -20C |
Sample Resuspension: | Samples were re-suspended in 620 ?l buffer (100 mM potassium phosphate [pH 7.3], 0.1% sodium azide, 1 mM trimethylsilylproprionate [TMSP] in D2O) |
Sample Spiking: | 6 internal standards (proline, ?-alanine, tyrosine, histidine, phosphocholine, and 3-hydroxykynurenine) added: 6 ?L of standards were added to a concentration of 2x the Chenomx-determined concentration for each metabolite |
Analysis:
MB Sample ID: | SA005626 |
Analysis ID: | AN000170 |
Laboratory Name: | Molecular Science Research Center (MSRC), Claflin University, Orangeburg, SC |
Analysis Type: | NMR |
Analysis Comments: | NMR: 2D 1H-13C HSQC |
Acquisition Date: | Summer 2012 |
Acquisition Parameters File: | nmr_data_acquisition_and_processing_protocol.doc |
Instrument Parameters File: | Cold_adaptation_in_Drosophila_melanogaster.zip |
Software Version: | TOPSPIN 2.1 |
Operator Name: | Miki Watanabe and Arezue Boroujerdi |
Chromatography ID: | CH000116 |
Num Factors: | 12 |
MB Sample ID: | SA005626 |
Analysis ID: | AN000169 |
Laboratory Name: | Molecular Science Research Center (MSRC), Claflin University, Orangeburg, SC |
Analysis Type: | NMR |
Analysis Comments: | NMR: 1D presaturation 1H |
Acquisition Date: | Summer 2012 |
Acquisition Parameters File: | nmr_data_acquisition_and_processing_protocol.doc |
Instrument Parameters File: | Cold_adaptation_in_Drosophila_melanogaster.zip |
Software Version: | TOPSPIN 2.1 |
Operator Name: | Miki Watanabe and Arezue Boroujerdi |
Chromatography ID: | CH000116 |
Num Factors: | 12 |
Num Metabolites: | 39 |
NMR:
NMR ID: | NM000036 |
Analysis ID: | AN000169 |
Instrument Name: | Bruker Avance III |
Instrument Type: | FT-NMR |
NMR Experiment Type: | 1D 1H |
Field Frequency Lock: | -6958.1 |
Standard Concentration: | 1 mM TMSP |
Spectrometer Frequency: | 700 MHz |
NMR Probe: | Room Temperature 5-mm triple resonance probe |
NMR Solvent: | D2O |
NMR Tube Size: | 5 mm |
Shimming Method: | topshim (automatic shimming) |
Pulse Sequence: | noesypr1d |
Water Suppression: | presaturation |
Pulse Width: | 9.4-9.6 ?s |
Power Level: | -1.00 dB |
Receiver Gain: | 200 |
Offset Frequency: | 3294.98 Hz |
Presaturation Power Level: | 53.64 dB |
Chemical Shift Ref Cpd: | TMSP |
Temperature: | 298 K |
Number Of Scans: | 120 |
Dummy Scans: | 4 |
Acquisition Time: | 2.9 s |
Relaxation Delay: | 4 s |
Spectral Width: | 16.0872 ppm |
Num Data Points Acquired: | 65536 |
Real Data Points: | 32768 |
Line Broadening: | 0.3 Hz |
Zero Filling: | 2x |
Apodization: | exponential multiply (em) |
Baseline Correction Method: | abs (automatic baseline correction with integration (1D)) |
Chemical Shift Ref Std: | 0.00 ppm |
NMR ID: | NM000037 |
Analysis ID: | AN000170 |
Instrument Name: | Bruker Avance III |
Instrument Type: | FT-NMR |
NMR Experiment Type: | 2D 1H-13C HSQC |
Field Frequency Lock: | -6958.1 |
Standard Concentration: | 1 mM TMSP |
Spectrometer Frequency: | 700 MHz |
NMR Probe: | Room Temperature 5-mm triple resonance probe |
NMR Solvent: | D2O |
NMR Tube Size: | 5 mm |
Shimming Method: | topshim (automatic shimming) |
Pulse Sequence: | HSQCEDETGPSISP2.2 |
Pulse Width: | 9.4-9.6 ?s |
Power Level: | f1 -1.00 dB, f2 -4.13 dB |
Receiver Gain: | 2050 |
Offset Frequency: | f2 3294.95 Hz, f1 14081.41 Hz |
Chemical Shift Ref Cpd: | TMSP |
Temperature: | 298 K |
Number Of Scans: | 256 |
Dummy Scans: | 256 |
Acquisition Time: | f2 0.13317 s, f1 0.0083333 s |
Relaxation Delay: | 1.5 s |
Spectral Width: | f2 10.9888, f1 180.00 |
Num Data Points Acquired: | f2 2048, f1 528 |
Real Data Points: | f2 4096, f1 2048 |
Line Broadening: | f2 1.0 Hz, f1 0.3 Hz |
Zero Filling: | f2 1x, f1 4x |
Apodization: | f2 QSINE, f1 QSINE |
Chemical Shift Ref Std: | 0.00, 0.00 ppm |