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MB Sample ID: SA005727
| Local Sample ID: | PLO_Met38 |
| Subject ID: | SU000123 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 40-76 |
| Weight Or Weight Range: | Normal, Overweight, Obese |
| Gender: | male , female |
| Human Smoking Status: | former |
| Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000123 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 40-76 |
| Weight Or Weight Range: | Normal, Overweight, Obese |
| Gender: | male , female |
| Human Smoking Status: | former |
| Species Group: | Human |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| PLO_Met38 | SA005727 | FL001490 | Male | gender |
| PLO_Met38 | SA005727 | FL001490 | Normal | bmi_cat |
| PLO_Met38 | SA005727 | FL001490 | low | drc_rank |
| PLO_Met38 | SA005727 | FL001490 | medium | methylation_rank |
| PLO_Met38 | SA005727 | FL001490 | <70 | Pack Years |
Collection:
| Collection ID: | CO000107 |
| Collection Summary: | - |
| Sample Type: | plasma |
| Volumeoramount Collected: | 1000uL |
| Storage Conditions: | -80 |
| Additives: | EDTA |
| Blood Serum Or Plasma: | plasma |
Treatment:
| Treatment ID: | TR000125 |
| Treatment Summary: | none |
Sample Preparation:
| Sampleprep ID: | SP000120 |
| Sampleprep Summary: | Aliquots of plasma samples (630 µL) were transferred into labeled tubes for experimental samples. Pooled high methylatiion, low methylation and combined plasma samples were prepared to serve as external QC samples. For all samples 70 µL of 10 mM formate solution (chemical shift indicator) in 0.9% saline in D2O with 0.02% NaN3 was added to each tube and were vortexed for 30 sec then centrifuged at 12000 rcf for 5 min. A 600 µL aliquot of the supernatant was transferred into 5 mm NMR tubes, which were kept on ice until data acquisition. |
| Processing Storage Conditions: | 4°C |
| Extract Storage: | -80C |
| Sample Resuspension: | D2O Saline |
| Sample Spiking: | formate |
Analysis:
| MB Sample ID: | SA005727 |
| Analysis ID: | AN000172 |
| Laboratory Name: | DHMRI |
| Analysis Type: | NMR |
| Acquisition Date: | 41597 |
| Software Version: | TopSpin 2.1 |
| Operator Name: | Kevin Knagge, Wimal Pathmasiri |
| Randomization Order: | Yes |
| Detector Type: | NMR |
| Data Format: | Bruker FID |
| Chromatography ID: | CH000118 |
| Num Factors: | 33 |
NMR:
| NMR ID: | NM000039 |
| Analysis ID: | AN000172 |
| Instrument Name: | Bruker Avance III |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D 1H |
| Field Frequency Lock: | D20 |
| Standard Concentration: | 1 mM |
| Spectrometer Frequency: | 700 MHz |
| NMR Probe: | 5mm Cryogenically Cooled ATMA |
| NMR Solvent: | D20 |
| NMR Tube Size: | 5mm |
| Shimming Method: | Topshim (Gradient) |
| Pulse Sequence: | cpmgpr1d |
| Water Suppression: | yes |
| Pulse Width: | 11.2 |
| Power Level: | 25.704 W |
| Receiver Gain: | 4.5 |
| Offset Frequency: | 3300.70 Hz |
| Chemical Shift Ref Cpd: | formate |
| Temperature: | 298 K |
| Number Of Scans: | 256 |
| Dummy Scans: | 4 |
| Acquisition Time: | 2.3243 |
| Relaxation Delay: | 2s |
| Spectral Width: | 20.13 ppm |
| Num Data Points Acquired: | 65536 |
| Real Data Points: | 65536 |
| Line Broadening: | 0.5 |
| Zero Filling: | Yes |
| Apodization: | Lorentzian |
| Baseline Correction Method: | Polynomial |
| Chemical Shift Ref Std: | Formate |
