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MB Sample ID: SA005968
Local Sample ID: | B02-10 |
Subject ID: | SU000126 |
Subject Type: | Animal |
Subject Species: | Mirounga angustirostris |
Taxonomy ID: | 9716 |
Species Group: | Mammal |
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Subject:
Subject ID: | SU000126 |
Subject Type: | Animal |
Subject Species: | Mirounga angustirostris |
Taxonomy ID: | 9716 |
Species Group: | Mammal |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
B02-10 | SA005968 | FL001536 | GLP1/Late GTT | Treatment |
B02-10 | SA005968 | FL001536 | 10 mins | Timepoint |
Collection:
Collection ID: | CO000110 |
Collection Summary: | Following the GLP-1 or insulin infusion, blood samples were collected at 10, 30, 60, 90, and 120 minutes. |
Collection Protocol Filename: | Newman_Lab-Endocannabinoid_Extraction_Analysis_Protocol-Ortiz_WCMC.pdf Newman_Lab_NEFA_SOPs-Ortiz_WCMC_Pilot.pdf |
Sample Type: | Blood |
Collection Time: | 10, 30, 60, 90, and 120 minutes postprandial |
Storage Conditions: | -80 C |
Blood Serum Or Plasma: | Plasma |
Treatment:
Treatment ID: | TR000128 |
Treatment Summary: | Seventeen northern elephant seal pups constituting four different cohorts at Año Nuevo State Reserve were studied at two post-weaning periods: early (1-2 wk post weaning; n=5) and late (6-7 weeks post weaning; n=12). Study #1: Prior to each infusion protocol, plasma U/kg). Following the infusion, blood samples were collected at 10,To determine the effects of prolonged fasting on peripheral insulin activity and function, samples were collected. Ten fasting seal pups (n=5 early, n=5 late) were infused (i.v.) with a mass-specific dose of insulin (0.065 U/kg). Following the infusion, blood samples were collected at 10, 30, 60, 90, and 120 minutes. Study #2: Seven, late-fasted seal pups were administered either a low (LDG; 10 pmol/kg; n=3) or high (HDG; 100 pmol/kg; n=4) dose of GLP-1 immediately following a glucose bolus (0.5 g/kg) (i.v.) infused within 2 mins. the infusions, blood samples were collected at 10, 30, 60, 90, 120, and 150 minutes. |
Treatment Protocol Filename: | Newman_Lab-Endocannabinoid_Extraction_Analysis_Protocol-Ortiz_WCMC.pdf Newman_Lab_NEFA_SOPs-Ortiz_WCMC_Pilot.pdf |
Treatment Compound: | GLP-1 or Insulin |
Treatment Route: | Infusion |
Treatment Dose: | low (10 pmol/kg) or high (100 pmol/kg) dose of GLP-1, insulin (0.065 U/kg) |
Animal Fasting: | Yes, for insulin study |
Sample Preparation:
Sampleprep ID: | SP000123 |
Sampleprep Summary: | - |
Sampleprep Protocol Filename: | Newman_Lab-Endocannabinoid_Extraction_Analysis_Protocol-Ortiz_WCMC.pdf Newman_Lab_NEFA_SOPs-Ortiz_WCMC_Pilot.pdf |
Processing Storage Conditions: | - 20 °C |
Extraction Method: | SPE |
Extract Concentration Dilution: | 250 µL |
Extract Cleanup: | SPE |
Extract Storage: | - 20 °C |
Sample Resuspension: | 100 µL |
Sample Spiking: | See sample prep protocol file |
Combined analysis:
Analysis ID | AN000177 | AN000178 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | GC | GC |
Chromatography system | ||
Column | ||
MS Type | ESI | EI |
MS instrument type | Triple quadrupole | Single quadrupole |
MS instrument name | ABI Sciex API 4000 QTrap | Agilent 5973N |
Ion Mode | POSITIVE | POSITIVE |
Units | nM | nM |
Chromatography:
Chromatography ID: | CH000121 |
Methods Filename: | Newman_Lab-Endocannabinoid_Extraction_Analysis_Protocol-Ortiz_WCMC.pdf Newman_Lab_NEFA_SOPs-Ortiz_WCMC_Pilot.pdf |
Column Temperature: | 60 C |
Flow Gradient: | See protocol/methods file |
Flow Rate: | 0.25 |
Internal Standard: | See protocol/methods file |
Retention Time: | See protocol/methods file |
Sample Injection: | 5 L |
Solvent A: | 100% water; 0.1% acetic acid |
Solvent B: | 90% acetonitrile/ 10% isopropanol |
Analytical Time: | 11 min |
Weak Wash Solvent Name: | 20% methanol, 10% isopropanol |
Weak Wash Volume: | 600 L |
Strong Wash Solvent Name: | 50:50 Acetonitrile:Methanol |
Strong Wash Volume: | 600 L |
Sample Loop Size: | 17 L |
Chromatography Type: | GC |
MS:
MS ID: | MS000140 |
Analysis ID: | AN000177 |
Instrument Name: | ABI Sciex API 4000 QTrap |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
MS Comments: | LC/MS |
Ion Mode: | POSITIVE |
Ion Source Temperature: | See protocol/methods file |
Ion Spray Voltage: | See protocol/methods file |
MS ID: | MS000141 |
Analysis ID: | AN000178 |
Instrument Name: | Agilent 5973N |
Instrument Type: | Single quadrupole |
MS Type: | EI |
MS Comments: | LC/MS |
Ion Mode: | POSITIVE |
Ion Source Temperature: | See protocol/methods file |
Ion Spray Voltage: | See protocol/methods file |