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MB Sample ID: SA007419
Local Sample ID: | 604 Jejunum |
Subject ID: | SU000154 |
Subject Type: | Animal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6J |
Age Or Age Range: | 6 weeks old |
Gender: | Males |
Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000154 |
Subject Type: | Animal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6J |
Age Or Age Range: | 6 weeks old |
Gender: | Males |
Species Group: | Mammal |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
604 Jejunum | SA007419 | FL001883 | Male | gender |
604 Jejunum | SA007419 | FL001883 | Tcdf Treated | genotype |
Collection:
Collection ID: | CO000138 |
Collection Summary: | - |
Sample Type: | Feces, liver, small and large intestine |
Treatment:
Treatment ID: | TR000156 |
Treatment Summary: | Wild type and AHR knock out mice (5-6 per group) were treated with TCDF for 5 days |
Treatment: | Dietary |
Treatment Compound: | TCDF |
Treatment Route: | Oral |
Treatment Dose: | 24 µg/kg body weight |
Sample Preparation:
Sampleprep ID: | SP000151 |
Sampleprep Summary: | - |
Sampleprep Protocol Filename: | Bile_Acid_Extraction_for_LCMS.docx |
Processing Method: | Homogenization |
Processing Storage Conditions: | -20 |
Extraction Method: | Ethanol extraction |
Sample Spiking: | Used D4 standards of Cholic Acid , Lithocholic Acid, Ursodeoxycholic Acid, Chenodeoxycholic Acid, and Deoxycholic Acid |
Organ: | Liver, small and large intestine, and feces |
Combined analysis:
Analysis ID | AN000217 |
---|---|
Analysis type | MS |
Chromatography type | |
Chromatography system | |
Column | |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | Waters |
Ion Mode | POSITIVE |
Units | Peak area |
Chromatography:
Chromatography ID: | CH000149 |
MS:
MS ID: | MS000169 |
Analysis ID: | AN000217 |
Instrument Name: | Waters |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
Ion Mode: | POSITIVE |