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MB Sample ID: SA007494
Local Sample ID: | P138 |
Subject ID: | SU000156 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000156 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Species Group: | Human |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
P138 | SA007494 | FL001890 | Patient | Sample Group |
Collection:
Collection ID: | CO000140 |
Collection Summary: | - |
Sample Type: | Serum |
Treatment:
Treatment ID: | TR000158 |
Treatment Summary: | None |
Sample Preparation:
Sampleprep ID: | SP000153 |
Sampleprep Summary: | Serum samples were analyzed following filtration through a 3-kD nominal cut-off membrane (Amicon Ultra 0.5 mL centrifugal filters, Millipore Corp, Billerica, MA) to remove proteins, lipids, lipoproteins, and protein-bound forms of certain molecules. Each serum sample was transferred to a prewashed filter and centrifuged at 13,800 g for 60 minutes as previously described (Gregory, Park et al. 2013). The filters were further washed with deuterium oxide (D2O) and the filtrate was mixed with the filtered serum in a 1:1 ratio. A reference buffer solution containing 5 mmol/L disodium-2,2-dimethyl 2-silapentane-5-sulphonate (DSS) and 10 mmol/L imidazole in D2O was added to the samples in a 1:9 ratio. The D2O provided a field-frequency lock, whereas DSS was used as the chemical shift reference and imidazole as pH indicator for the NMR spectra. A total of 600 µL mixture was transferred to a 5 mm NMR tube (Sigma Aldrich, St. Louis, MO). |
Analysis:
MB Sample ID: | SA007494 |
Analysis ID: | AN000219 |
Analysis Type: | NMR |
Chromatography ID: | CH000151 |
Num Factors: | 2 |
Num Metabolites: | 33 |
NMR:
NMR ID: | NM000051 |
Analysis ID: | AN000219 |
Instrument Name: | Agilent |
Instrument Type: | FT-NMR |
NMR Experiment Type: | NOESY |
Spectrometer Frequency: | 600 MHz |