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MB Sample ID: SA008889
| Local Sample ID: | VT_120218_016 |
| Subject ID: | SU000182 |
| Subject Type: | Human/animal |
| Subject Species: | Homo sapiens;Callithrix jacchus |
| Taxonomy ID: | 9606;9483 |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000182 |
| Subject Type: | Human/animal |
| Subject Species: | Homo sapiens;Callithrix jacchus |
| Taxonomy ID: | 9606;9483 |
| Species Group: | Mammal |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| VT_120218_016 | SA008889 | FL002128 | Marmoset | species |
Collection:
| Collection ID: | CO000168 |
| Collection Summary: | - |
Treatment:
| Treatment ID: | TR000188 |
| Treatment Summary: | No treatment |
Sample Preparation:
| Sampleprep ID: | SP000182 |
| Sampleprep Summary: | aliquots of 65ul sample were mixed with 130ul acetonitrile and 3.25ul internal standard. Vortex for 1 minute on high and leave on ice for 30 minutes. Centrifuge for 10 minutes at 13.2rpm. 130ul of supernatant was removed and placed into autosampler vials for the mass spectrometer. |
| Processing Method: | precipitation of protein, centrifuge, and remove supernatant |
| Processing Storage Conditions: | on ice |
| Extraction Method: | 1:2 sample:acetonitrile |
| Extract Storage: | supernatant pipette into autosampler vials for mass spectrometer |
| Sample Spiking: | internal standards: [15N,13C5]-L-methionine, [2-15N]-L-lysine dihydrochloride, [15N]-choline chloride, [13C5]-L-glutamic acid, [13C7]-benzoic acid, [15N]-L-tyrosine, [15N2]-uracil, [3,3-13C2]-cystine, [trimethyl-13C3]-caffeine, 2'-deoxyguanosine-13C10,15N5-5'-monophosphate |
Combined analysis:
| Analysis ID | AN000255 |
|---|---|
| Analysis type | MS |
| Chromatography type | Anion exchange/Reversed phase |
| Chromatography system | Thermo Accela CTC |
| Column | Higgins C18 (100 x 2.1mm,5um); Hamilton Anion Exchange 100x2.1mm,7um |
| MS Type | ESI |
| MS instrument type | Single quadrupole |
| MS instrument name | Thermo LTQ |
| Ion Mode | POSITIVE |
| Units | Intensity |
Chromatography:
| Chromatography ID: | CH000180 |
| Chromatography Summary: | untargeted dual chromatography (C18 and Anion Exchange column) |
| Methods ID: | Methods_LTQVelosOrbi |
| Methods Filename: | Methods_LTQVelosOrbi |
| Instrument Name: | Thermo Accela CTC |
| Column Name: | Higgins C18 (100 x 2.1mm,5um); Hamilton Anion Exchange 100x2.1mm,7um |
| Column Pressure: | 200 Bar (max) |
| Column Temperature: | 30 |
| Flow Gradient: | C18: A% = 5% for 10 minutes, 0-2mins = 60% B, 2-6mins = gradient to 95% B, 6-10mins = 95% B; AE: 1-10mins = 50%B, 0-2mins = 5%A, 2-7mins = gradient to 50%A, 7-10mins = 50%A |
| Flow Rate: | 0.35 mL/min |
| Injection Temperature: | 4C |
| Sample Injection: | 10ul |
| Sampling Cone: | ESI |
| Solvent A: | 100% water; 0.2% formic acid |
| Solvent B: | 100% acetonitrile |
| Analytical Time: | LC grade water |
| Capillary Voltage: | 10 minutes |
| Migration Time: | 100uA (source current) |
| Sheath Liquid: | 4.5kV (source voltage) |
| Weak Wash Solvent Name: | water with 10% methanol |
| Sample Syringe Size: | 20ul |
| Chromatography Type: | Anion exchange/Reversed phase |
MS:
| MS ID: | MS000205 |
| Analysis ID: | AN000255 |
| Instrument Name: | Thermo LTQ |
| Instrument Type: | Single quadrupole |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Capillary Temperature: | 4.45 |
| Collision Energy: | N2 |
| Fragmentation Method: | 50psi |
| Ion Spray Voltage: | electrospray ionization |
| Ionization Potential: | 5ppm |
| Reagent Gas: | 48 |
| Atom Gun Current: | 1000000 |
| Cdl Temperature: | .raw |
| Sample Dripping: | 85-2000 |
