Return to study ST000163 main page
MB Sample ID: SA008913
Local Sample ID: | VT_120217_028 |
Subject ID: | SU000182 |
Subject Type: | Human/animal |
Subject Species: | Homo sapiens;Callithrix jacchus |
Taxonomy ID: | 9606;9483 |
Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000182 |
Subject Type: | Human/animal |
Subject Species: | Homo sapiens;Callithrix jacchus |
Taxonomy ID: | 9606;9483 |
Species Group: | Mammal |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
VT_120217_028 | SA008913 | FL002128 | Marmoset | species |
Collection:
Collection ID: | CO000168 |
Collection Summary: | - |
Treatment:
Treatment ID: | TR000188 |
Treatment Summary: | No treatment |
Sample Preparation:
Sampleprep ID: | SP000182 |
Sampleprep Summary: | aliquots of 65ul sample were mixed with 130ul acetonitrile and 3.25ul internal standard. Vortex for 1 minute on high and leave on ice for 30 minutes. Centrifuge for 10 minutes at 13.2rpm. 130ul of supernatant was removed and placed into autosampler vials for the mass spectrometer. |
Processing Method: | precipitation of protein, centrifuge, and remove supernatant |
Processing Storage Conditions: | on ice |
Extraction Method: | 1:2 sample:acetonitrile |
Extract Storage: | supernatant pipette into autosampler vials for mass spectrometer |
Sample Spiking: | internal standards: [15N,13C5]-L-methionine, [2-15N]-L-lysine dihydrochloride, [15N]-choline chloride, [13C5]-L-glutamic acid, [13C7]-benzoic acid, [15N]-L-tyrosine, [15N2]-uracil, [3,3-13C2]-cystine, [trimethyl-13C3]-caffeine, 2'-deoxyguanosine-13C10,15N5-5'-monophosphate |
Combined analysis:
Analysis ID | AN000255 |
---|---|
Analysis type | MS |
Chromatography type | Anion exchange/Reversed phase |
Chromatography system | Thermo Accela CTC |
Column | Higgins C18 (100 x 2.1mm,5um); Hamilton Anion Exchange 100x2.1mm,7um |
MS Type | ESI |
MS instrument type | Single quadrupole |
MS instrument name | Thermo LTQ |
Ion Mode | POSITIVE |
Units | Intensity |
Chromatography:
Chromatography ID: | CH000180 |
Chromatography Summary: | untargeted dual chromatography (C18 and Anion Exchange column) |
Methods ID: | Methods_LTQVelosOrbi |
Methods Filename: | Methods_LTQVelosOrbi |
Instrument Name: | Thermo Accela CTC |
Column Name: | Higgins C18 (100 x 2.1mm,5um); Hamilton Anion Exchange 100x2.1mm,7um |
Column Pressure: | 200 Bar (max) |
Column Temperature: | 30 |
Flow Gradient: | C18: A% = 5% for 10 minutes, 0-2mins = 60% B, 2-6mins = gradient to 95% B, 6-10mins = 95% B; AE: 1-10mins = 50%B, 0-2mins = 5%A, 2-7mins = gradient to 50%A, 7-10mins = 50%A |
Flow Rate: | 0.35 mL/min |
Injection Temperature: | 4C |
Sample Injection: | 10ul |
Sampling Cone: | ESI |
Solvent A: | 100% water; 0.2% formic acid |
Solvent B: | 100% acetonitrile |
Analytical Time: | LC grade water |
Capillary Voltage: | 10 minutes |
Migration Time: | 100uA (source current) |
Sheath Liquid: | 4.5kV (source voltage) |
Weak Wash Solvent Name: | water with 10% methanol |
Sample Syringe Size: | 20ul |
Chromatography Type: | Anion exchange/Reversed phase |
MS:
MS ID: | MS000205 |
Analysis ID: | AN000255 |
Instrument Name: | Thermo LTQ |
Instrument Type: | Single quadrupole |
MS Type: | ESI |
Ion Mode: | POSITIVE |
Capillary Temperature: | 4.45 |
Collision Energy: | N2 |
Fragmentation Method: | 50psi |
Ion Spray Voltage: | electrospray ionization |
Ionization Potential: | 5ppm |
Reagent Gas: | 48 |
Atom Gun Current: | 1000000 |
Cdl Temperature: | .raw |
Sample Dripping: | 85-2000 |