Return to study ST000220 main page

MB Sample ID: SA010725

Local Sample ID:SF-100109-130034
Subject ID:SU000239
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Cell Biosource Or Supplier:Moffitt Cancer Center Tissue Core
Species Group:Human

Select appropriate tab below to view additional metadata details:

Subject:

Subject ID:SU000239
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Cell Biosource Or Supplier:Moffitt Cancer Center Tissue Core
Species Group:Human

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
SF-100109-130034SA010725FL002551Snap FrozenHistology Type Conformed - CAP

Collection:

Collection ID:CO000227
Collection Summary:-
Sample Type:tissue
Collection Method:Snap frozen

Treatment:

Treatment ID:TR000247

Sample Preparation:

Sampleprep ID:SP000241
Sampleprep Summary:Pulverized tissue samples were incubated in 50:50 Acetonitrile:Water for 1 hr, vortexed and centrifuged. Supernatants were transferred to new tubes along with isotopocially labled internal standard and samples were lyophilized to dryness. Dried samples were resuspended in 95:5 Water:Methanol and an aliquot was transferred to autosampler vials forbroad spectrum metabolomics data analysis acquisition.
Processing Method:Pulverization of frozen tissue (performed at Moffitt and supplied to RTI)
Extraction Method:50:50 Acetonitrile:Water
Extract Storage:-80 C
Sample Resuspension:95:5 Water:Methanol
Sample Spiking:L-Tryptophan-d5
Organ:Lung

Combined analysis:

Analysis ID AN000325 AN000326
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Waters Acquity Waters Acquity
Column Waters Acquity HSS T3 (100 x 2.1mm,1.8um) Waters Acquity HSS T3 (100 x 2.1mm,1.8um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Waters Synapt-G2-Si Waters Synapt-G2-Si
Ion Mode POSITIVE NEGATIVE
Units Normalized abundance Normalized abundance

Chromatography:

Chromatography ID:CH000245
Instrument Name:Waters Acquity
Column Name:Waters Acquity HSS T3 (100 x 2.1mm,1.8um)
Column Pressure:6000-10000
Column Temperature:50 C
Flow Gradient:Time(min) Flow Rate %A %B Curve; 1. Initial 0.400 100.0 0.0; 2. 1.00 0.400 100.0 0.0 6;3. 16.00 0.400 0.0 100.0 6; 4. 20.00 0.400 0.0 100.0 6; 5. 22.00 0.400 100.0 0.0 6
Flow Rate:0.4 mL/min
Injection Temperature:8 C
Internal Standard:L-Tryptophan-d5
Solvent A:100% water; 0.1% formic acid
Solvent B:100% methanol; 0.1% formic acid
Analytical Time:22 min
Weak Wash Solvent Name:5%MeOH
Weak Wash Volume:1000 uL
Strong Wash Solvent Name:80%MeOH
Strong Wash Volume:1000 uL
Target Sample Temperature:8 C
Sample Loop Size:10 uL
Sample Syringe Size:100 uL
Randomization Order:Yes
Chromatography Type:Reversed phase

MS:

MS ID:MS000274
Analysis ID:AN000325
Instrument Name:Waters Synapt-G2-Si
Instrument Type:QTOF
MS Type:ESI
Ion Mode:POSITIVE
Capillary Temperature:110 C
Capillary Voltage:1.0 kV
Collision Energy:4
Fragmentation Method:CID
Helium Flow:180
Ionization:ES+
Mass Accuracy:5 ppm
Source Temperature:110 C
Dataformat:Continuum
Desolvation Gas Flow:400 L/Hr
Desolvation Temperature:400 C
Resolution Setting:18000
Scan Range Moverz:50-1000 m/s
Scanning Cycle:1 s
Tube Lens Voltage:75
  
MS ID:MS000275
Analysis ID:AN000326
Instrument Name:Waters Synapt-G2-Si
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
Capillary Temperature:110 C
Capillary Voltage:1.0 kV
Collision Energy:4
Fragmentation Method:CID
Helium Flow:180
Ionization:ES-
Mass Accuracy:5 ppm
Source Temperature:110 C
Dataformat:Continuum
Desolvation Gas Flow:400 L/Hr
Desolvation Temperature:400 C
Resolution Setting:18000
Scan Range Moverz:50-1000 m/z
Scanning Cycle:1 s
Tube Lens Voltage:74
  logo