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MB Sample ID: SA010735

Local Sample ID:SF-100109-142930
Subject ID:SU000239
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Cell Biosource Or Supplier:Moffitt Cancer Center Tissue Core
Species Group:Human

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Subject:

Subject ID:SU000239
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Cell Biosource Or Supplier:Moffitt Cancer Center Tissue Core
Species Group:Human

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
SF-100109-142930SA010735FL002561Small cell carcinoma, NOSHistology Type Conformed - CAP

Collection:

Collection ID:CO000227
Collection Summary:-
Sample Type:tissue
Collection Method:Snap frozen

Treatment:

Treatment ID:TR000247

Sample Preparation:

Sampleprep ID:SP000241
Sampleprep Summary:Pulverized tissue samples were incubated in 50:50 Acetonitrile:Water for 1 hr, vortexed and centrifuged. Supernatants were transferred to new tubes along with isotopocially labled internal standard and samples were lyophilized to dryness. Dried samples were resuspended in 95:5 Water:Methanol and an aliquot was transferred to autosampler vials forbroad spectrum metabolomics data analysis acquisition.
Processing Method:Pulverization of frozen tissue (performed at Moffitt and supplied to RTI)
Extraction Method:50:50 Acetonitrile:Water
Extract Storage:-80 C
Sample Resuspension:95:5 Water:Methanol
Sample Spiking:L-Tryptophan-d5
Organ:Lung

Combined analysis:

Analysis ID AN000325 AN000326
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Waters Acquity Waters Acquity
Column Waters Acquity HSS T3 (100 x 2.1mm,1.8um) Waters Acquity HSS T3 (100 x 2.1mm,1.8um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Waters Synapt G2 Si QTOF Waters Synapt G2 Si QTOF
Ion Mode POSITIVE NEGATIVE
Units Normalized abundance Normalized abundance

Chromatography:

Chromatography ID:CH000245
Instrument Name:Waters Acquity
Column Name:Waters Acquity HSS T3 (100 x 2.1mm,1.8um)
Column Pressure:6000-10000
Column Temperature:50 C
Flow Gradient:Time(min) Flow Rate %A %B Curve; 1. Initial 0.400 100.0 0.0; 2. 1.00 0.400 100.0 0.0 6;3. 16.00 0.400 0.0 100.0 6; 4. 20.00 0.400 0.0 100.0 6; 5. 22.00 0.400 100.0 0.0 6
Flow Rate:0.4 mL/min
Injection Temperature:8 C
Internal Standard:L-Tryptophan-d5
Solvent A:100% water; 0.1% formic acid
Solvent B:100% methanol; 0.1% formic acid
Analytical Time:22 min
Weak Wash Solvent Name:5%MeOH
Weak Wash Volume:1000 uL
Strong Wash Solvent Name:80%MeOH
Strong Wash Volume:1000 uL
Target Sample Temperature:8 C
Sample Loop Size:10 uL
Sample Syringe Size:100 uL
Randomization Order:Yes
Chromatography Type:Reversed phase

MS:

MS ID:MS000274
Analysis ID:AN000325
Instrument Name:Waters Synapt G2 Si QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:POSITIVE
Capillary Temperature:110 C
Capillary Voltage:1.0 kV
Collision Energy:4
Fragmentation Method:CID
Helium Flow:180
Ionization:ES+
Mass Accuracy:5 ppm
Source Temperature:110 C
Dataformat:Continuum
Desolvation Gas Flow:400 L/Hr
Desolvation Temperature:400 C
Resolution Setting:18000
Scan Range Moverz:50-1000 m/s
Scanning Cycle:1 s
Tube Lens Voltage:75
  
MS ID:MS000275
Analysis ID:AN000326
Instrument Name:Waters Synapt G2 Si QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
Capillary Temperature:110 C
Capillary Voltage:1.0 kV
Collision Energy:4
Fragmentation Method:CID
Helium Flow:180
Ionization:ES-
Mass Accuracy:5 ppm
Source Temperature:110 C
Dataformat:Continuum
Desolvation Gas Flow:400 L/Hr
Desolvation Temperature:400 C
Resolution Setting:18000
Scan Range Moverz:50-1000 m/z
Scanning Cycle:1 s
Tube Lens Voltage:74
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