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MB Sample ID: SA010752
| Local Sample ID: | SVM0322 |
| Subject ID: | SU000242 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000242 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| SVM0322 | SA010752 | FL002568 | Case | Type |
Collection:
| Collection ID: | CO000230 |
| Collection Summary: | All participants have been carefully phenotyped and have a molecular confirmation of BTHS. In order to mitigate the potential effects of intercurrent illness in individual patients, plasma samples were collected under controlled conditions, while the patient is in a homeostatic state of wellness (outpatient setting). We controlled for nutritional effects by selecting samples hta thave amino acid profiles consistent with 4-6 hours of fasting. All samples were collected in an EDTA treated collection tube, plasma was separated via centrifugation and has been stored in a controlled setting at -80 degrees Celsius since collection. |
| Sample Type: | Blood |
Treatment:
| Treatment ID: | TR000250 |
| Treatment Summary: | none |
Sample Preparation:
| Sampleprep ID: | SP000244 |
| Sampleprep Summary: | Aliquots of plasma samples (125 µL) were transferred into pre-labeled 2.0 mL low-bind Eppendorf tubes for experimental samples. For all samples 125 µL of 0.9% Saline in D2O (containing 2 mM Formate (chemical shift indicator) and 0.2% NaN3 (to prevent bacterial growth)) was added to each tube and were vortexed for 2 min at 5000 rpm. The samples were then centrifuged at 16,000 rcf for 2 min. Total pools of the samples were prepared (by using 12 µL of each study sample) to serve as QC samples, and 125 µL aliquots of QC samples were processed in the same way as described above for study samples. A 200 µL aliquot of the samples were then transferred into 3 mm NMR tubes (Bruker-Biospin, Switzerland), which were kept in a cooler with freeze packs until data acquisition. |
| Processing Storage Conditions: | 4°C |
| Sample Resuspension: | 0.9% Saline in D2O |
| Sample Spiking: | Formate |
Analysis:
| MB Sample ID: | SA010752 |
| Analysis ID: | AN000332 |
| Laboratory Name: | DHMRI |
| Analysis Type: | NMR |
| Software Version: | Top Spin 3.2 |
| Operator Name: | Keven Knagge, Wimal Pathmasiri |
| Randomization Order: | Yes |
| Detector Type: | NMR |
| Data Format: | Bruker FID |
| Chromatography ID: | CH000249 |
| Num Factors: | 3 |
NMR:
| NMR ID: | NM000053 |
| Analysis ID: | AN000332 |
| Instrument Name: | Bruker Avance III |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D 1H |
| Pulse Sequence: | Yes |
| Pulse Width: | 41967 |
| Power Level: | Deuterium |
| Receiver Gain: | 1 mM (Formate) |
| Offset Frequency: | 950 MHz |
| Presaturation Power Level: | 5mm Cryogenically Cooled ATMA |
| Chemical Shift Ref Cpd: | D20 |
| Temperature: | 5mm |
| Number Of Scans: | Topshim (Gradient) |
| Dummy Scans: | cpmgpr1d |
| Acquisition Time: | yes |
| Relaxation Delay: | 8.11 |
| Spectral Width: | 12.589 W |
| Num Data Points Acquired: | 4 |
| Real Data Points: | 4468.1 Hz |
| Zero Filling: | Formate |
| Apodization: | 298 K |
| Baseline Correction Method: | 128 |
| Chemical Shift Ref Std: | 16 |
