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MB Sample ID: SA010882
| Local Sample ID: | MAL2 |
| Subject ID: | SU000251 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6 |
| Age Or Age Range: | 6 weeks at collection |
| Gender: | Female |
| Animal Animal Supplier: | Jackson Labs |
| Animal Housing: | Conventional |
| Animal Feed: | Low Protein, Low Fat Malnourished Diet|Isocaloric Control Diet |
| Subject Comments: | 2 subjects |
| Cell Primary Immortalized: | Malnourished|Control |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000251 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6 |
| Age Or Age Range: | 6 weeks at collection |
| Gender: | Female |
| Animal Animal Supplier: | Jackson Labs |
| Animal Housing: | Conventional |
| Animal Feed: | Low Protein, Low Fat Malnourished Diet|Isocaloric Control Diet |
| Subject Comments: | 2 subjects |
| Cell Primary Immortalized: | Malnourished|Control |
| Species Group: | Mammal |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| MAL2 | SA010882 | FL002645 | malnourished | Diet |
Collection:
| Collection ID: | CO000241 |
| Collection Summary: | - |
| Sample Type: | Small intestinal fecal contents |
| Collection Time: | 6 weeks of age |
| Storage Conditions: | -80 degrees |
| Tissue Cell Quantity Taken: | 15-40mg |
Treatment:
| Treatment ID: | TR000261 |
| Treatment Summary: | 4 mice were given a malnourished diet and 4 mice were untreated, fed a control diet |
Sample Preparation:
| Sampleprep ID: | SP000255 |
| Sampleprep Summary: | Metabolite Extraction. Mouse small intestine digesta samples (luminal contents) were collected, weighed and homogenized in water followed by addition of acetonitrile for metabolite extraction. After vortex mixing, 30 second sonication, and centrifugation, the clear supernatants were collected and dried under a gentle nitrogen gas flow. The dried metabolite residues were dissolved in 40% acetonitrile containing 0.01% formic acid, 4 ?L per mg of the raw material. After 15 sec vortex and 30 sec sonication, each sample was further diluted 1:2 with water. Twelve-?L aliquots were injected for untargeted metabolic fingerprinting by ultrahigh-performance liquid chromatography ? Fourier transform mass spectrometry (UPLC-FTMS). |
Combined analysis:
| Analysis ID | AN000347 | AN000348 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | ||
| Column | C18 | C18 |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Relative Abundance | Relative Abundance |
Chromatography:
| Chromatography ID: | CH000259 |
| Column Name: | C18 |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000293 |
| Analysis ID: | AN000347 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| MS ID: | MS000294 |
| Analysis ID: | AN000348 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
