Return to study ST000243 main page
MB Sample ID: SA011230
Local Sample ID: | EXP_2_0.5MIC_1 |
Subject ID: | SU000263 |
Subject Type: | Yeast cells |
Subject Species: | Candida albicans |
Taxonomy ID: | 5476 |
Genotype Strain: | SC5314 |
Species Group: | Microorganism |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000263 |
Subject Type: | Yeast cells |
Subject Species: | Candida albicans |
Taxonomy ID: | 5476 |
Genotype Strain: | SC5314 |
Species Group: | Microorganism |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
EXP_2_0.5MIC_1 | SA011230 | FL002732 | 0.5MIC | Treatment |
EXP_2_0.5MIC_1 | SA011230 | FL002732 | 2 | experiment |
Collection:
Collection ID: | CO000254 |
Collection Summary: | Culture supernatants (appx. 1 ml from each replicate) will be removed and stored at -80C |
Sample Type: | Cells |
Volumeoramount Collected: | 1 mL |
Storage Conditions: | -80C |
Treatment:
Treatment ID: | TR000274 |
Treatment Summary: | Candida cells grown to the mid-logarithmic phase will be exposed in biological replicates (X8) to the antifungal agent (micafungin) to a range of concentrations (sub-concentrations of the strain's MIC, Minimal Inhibitory Concentration) 0.0625MIC 0.125MIC 0.25MIC 0.5MIC 1.0MIC. No drug exposed Candida cells will serve as controls |
Sample Preparation:
Sampleprep ID: | SP000268 |
Sampleprep Summary: | - |
Sampleprep Protocol Filename: | Sample_Preparation_of_Cell_Lysates.pdf |
Combined analysis:
Analysis ID | AN000379 | AN000380 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | Peak height | Peak height |
Chromatography:
Chromatography ID: | CH000273 |
Methods Filename: | Metabolomics_LCMSProtocol.pdf Appendix_A_-_Internal_Standard_Prep_GLCMS.pdf |
Instrument Name: | Thermo Dionex Ultimate 3000 |
Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
Flow Rate: | 0.350mL/min-0.600mL/min |
Internal Standard: | Appendix A - Internal Standard Prep GLCMS.pdf |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS000325 |
Analysis ID: | AN000379 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
Ion Mode: | POSITIVE |
MS ID: | MS000326 |
Analysis ID: | AN000380 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
Ion Mode: | NEGATIVE |