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MB Sample ID: SA012101
Local Sample ID: | QE1_REP_18_23 |
Subject ID: | SU000289 |
Subject Type: | Animal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6 |
Age Or Age Range: | 8 or 24 week |
Gender: | male |
Animal Animal Supplier: | Jackson Laboratories |
Animal Feed: | Control (Research Diets, Inc. # D09100304) and trans-fat diet (Research Diets, Inc. # D09100301) |
Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000289 |
Subject Type: | Animal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6 |
Age Or Age Range: | 8 or 24 week |
Gender: | male |
Animal Animal Supplier: | Jackson Laboratories |
Animal Feed: | Control (Research Diets, Inc. # D09100304) and trans-fat diet (Research Diets, Inc. # D09100301) |
Species Group: | Mammal |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
QE1_REP_18_23 | SA012101 | FL003019 | 8 | Age (weeks) |
QE1_REP_18_23 | SA012101 | FL003019 | Control | Diet |
Collection:
Collection ID: | CO000283 |
Collection Summary: | - |
Sample Type: | tissue (liver) |
Collection Method: | tissue flash frozen in liquid nitrogen immediately after sacrifice |
Treatment:
Treatment ID: | TR000303 |
Treatment Summary: | Mice were either fed a control diet or high fructose trans-fat diet for 8 or 24 weeks |
Treatment Compound: | diet (Research Diets, Inc) |
Treatment Route: | oral |
Animal Anesthesia: | isoflurane (3% in O2) |
Animal Endp Clinical Signs: | accompanied histology |
Sample Preparation:
Sampleprep ID: | SP000297 |
Sampleprep Summary: | Tissues were cryogenically ground prior to beign weighed and homogenized in chloroform and methanol for the Folch extraction |
Processing Method: | homogenization (zirconium beads, 1.0 mm diameter), lipid extraction |
Processing Storage Conditions: | Liquid nitrogen and on ice |
Extraction Method: | Folch |
Extract Storage: | -80C |
Sample Resuspension: | isopropanol |
Sample Spiking: | Internal Standards: Cer(d18:1/17:0) and d5-DAG (20:2/20:2) and d5-DG (20:5/20:5) |
Organ: | liver |
Combined analysis:
Analysis ID | AN000431 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Thermo Dionex Ultimate 3000 |
Column | Waters Acquity BEH C18 (50 x 2.1mm,1.7um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE |
Units | nmol/g liver protein |
Chromatography:
Chromatography ID: | CH000301 |
Chromatography Summary: | Waters Acquity BEH C18 (2.1 x 50 mm, 1.7 um) with guard was used to separate compounds with mobile phases made of isopropanol, acetonitrile, and water. |
Methods Filename: | REP_18 |
Instrument Name: | Thermo Dionex Ultimate 3000 |
Column Name: | Waters Acquity BEH C18 (50 x 2.1mm,1.7um) |
Column Temperature: | 30 C |
Flow Gradient: | 32% B to 98% B over 18 mins |
Flow Rate: | 0.5 mL/min |
Injection Temperature: | 4 C |
Solvent A: | 40% water/60% acetonitrile; 0.1% formic acid; 10 mM ammonium formate |
Solvent B: | 90% isopropanol/10% acetonitrile; 0.1% formic acid; 10 mM ammonium formate |
Analytical Time: | 18 mins |
Capillary Voltage: | 350 C |
Time Program: | 0-1 min: 32%B --> 1-1.5 min: 40% B --> 4 min: 45% B --> 5 min: 50% B --> 8 min: 60% B --> 11 min: 70% B --> 14 min: 80% B --> 16-17 min: 100%B --> 18 min: 32%B --> 21 min: 32% B |
Strong Wash Solvent Name: | 1:1:1 isopropanol:acetonitrile:water |
Strong Wash Volume: | 100 uL |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS000372 |
Analysis ID: | AN000431 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
Ion Mode: | POSITIVE |
Capillary Temperature: | 300 |
Dry Gas Flow: | 30 |
Ion Source Temperature: | 350 |
Ion Spray Voltage: | 3500 |
Mass Accuracy: | 3 ppm |
Automatic Gain Control: | 3000000 |
Desolvation Temperature: | 350 |
Resolution Setting: | 17500 |
Scan Range Moverz: | 150-2000 |
Acquisition Parameters File: | REP_18 |
Processing Parameters File: | REP_18_sequence_processing |