Return to study ST000285 main page
MB Sample ID: SA012589
Local Sample ID: | 122 |
Subject ID: | SU000305 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Age Or Age Range: | 21-86 |
Weight Or Weight Range: | 45-172 kg |
Height Or Height Range: | 137-200 cm |
Gender: | 64 M and 63 F |
Human Ethnicity: | Indicated in the Study Design |
Human Smoking Status: | Indicated in the Study Design |
Human Alcohol Drug Use: | Indicated in the Study Design |
Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000305 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Age Or Age Range: | 21-86 |
Weight Or Weight Range: | 45-172 kg |
Height Or Height Range: | 137-200 cm |
Gender: | 64 M and 63 F |
Human Ethnicity: | Indicated in the Study Design |
Human Smoking Status: | Indicated in the Study Design |
Human Alcohol Drug Use: | Indicated in the Study Design |
Species Group: | Human |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
122 | SA012589 | FL003097 | Colorectal Cancer | Disease type |
Collection:
Collection ID: | CO000299 |
Collection Summary: | Serum samples from patients with CRC, polyps, and matched healthy controls were obtained from the Indiana University School of Medicine. Following the IRB protocol approved by both Indiana and Purdue Universities, patients undergoing colonoscopy for CRC screening were evaluated and blood from the consented patients was obtained after overnight fasting and bowel preparation but prior to colonoscopy. Blood samples were allowed to clot at room temperature for 45 min and then centrifuged at 2000 rpm for 10 min. The sera were collected, and aliquoted into separate vials, then transported to Purdue University over dry ice and stored at -80°C until used for analysis. |
Sample Type: | Blood |
Blood Serum Or Plasma: | Serum |
Treatment:
Treatment ID: | TR000319 |
Treatment Summary: | No treatment |
Human Fasting: | Overnight |
Sample Preparation:
Sampleprep ID: | SP000313 |
Sampleprep Summary: | Each frozen serum sample was thawed and vortexed; 530 µL aliquots were mixed with 5 µL sodium azide solution (5% in H2O). The resulting solution was centrifuged, and 530 µL was transferred to a 5 mm NMR tube. A coaxial capillary containing 60 µL TSP (20.9 nmol) in D2O was placed into the NMR tube to serve as a chemical shift and quantitative reference. The samples were randomized before performing the NMR experiments. |
Analysis:
MB Sample ID: | SA012589 |
Analysis ID: | AN000453 |
Analysis Type: | NMR |
Num Factors: | 3 |
NMR:
NMR ID: | NM000059 |
Analysis ID: | AN000453 |
Instrument Name: | Bruker Avance III |
Instrument Type: | FT-NMR |
NMR Experiment Type: | 1D 1H |
Spectrometer Frequency: | 500 MHz |
NMR Probe: | HCN cryogenic probe |
NMR Solvent: | D2O |
NMR Tube Size: | 5 mm |
Pulse Sequence: | CPMG, NOESY |
Chemical Shift Ref Cpd: | TSP |
Temperature: | 25C |
Number Of Scans: | 128 |
Spectral Width: | 6000 Hz |
Num Data Points Acquired: | 16K |
Line Broadening: | 0.5 Hz |
Binned Increment: | 0.00587 |