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MB Sample ID: SA013057

Local Sample ID:377
Subject ID:SU000305
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Age Or Age Range:21-86
Weight Or Weight Range:45-172 kg
Height Or Height Range:137-200 cm
Gender:64 M and 63 F
Human Ethnicity:Indicated in the Study Design
Human Smoking Status:Indicated in the Study Design
Human Alcohol Drug Use:Indicated in the Study Design
Species Group:Human

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Subject:

Subject ID:SU000305
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Age Or Age Range:21-86
Weight Or Weight Range:45-172 kg
Height Or Height Range:137-200 cm
Gender:64 M and 63 F
Human Ethnicity:Indicated in the Study Design
Human Smoking Status:Indicated in the Study Design
Human Alcohol Drug Use:Indicated in the Study Design
Species Group:Human

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
377SA013057FL003099PolypDisease type

Collection:

Collection ID:CO000299
Collection Summary:Serum samples from patients with CRC, polyps, and matched healthy controls were obtained from the Indiana University School of Medicine. Following the IRB protocol approved by both Indiana and Purdue Universities, patients undergoing colonoscopy for CRC screening were evaluated and blood from the consented patients was obtained after overnight fasting and bowel preparation but prior to colonoscopy. Blood samples were allowed to clot at room temperature for 45 min and then centrifuged at 2000 rpm for 10 min. The sera were collected, and aliquoted into separate vials, then transported to Purdue University over dry ice and stored at -80°C until used for analysis.
Sample Type:Blood
Blood Serum Or Plasma:Serum

Treatment:

Treatment ID:TR000319
Treatment Summary:No treatment
Human Fasting:Overnight

Sample Preparation:

Sampleprep ID:SP000313
Sampleprep Summary:Each frozen serum sample was thawed and vortexed; 530 µL aliquots were mixed with 5 µL sodium azide solution (5% in H2O). The resulting solution was centrifuged, and 530 µL was transferred to a 5 mm NMR tube. A coaxial capillary containing 60 µL TSP (20.9 nmol) in D2O was placed into the NMR tube to serve as a chemical shift and quantitative reference. The samples were randomized before performing the NMR experiments.

Analysis:

MB Sample ID:SA013057
Analysis ID:AN000453
Analysis Type:NMR
Num Factors:3

NMR:

NMR ID:NM000059
Analysis ID:AN000453
Instrument Name:Bruker Avance III
Instrument Type:FT-NMR
NMR Experiment Type:1D 1H
Spectrometer Frequency:500 MHz
NMR Probe:HCN cryogenic probe
NMR Solvent:D2O
NMR Tube Size:5 mm
Pulse Sequence:CPMG, NOESY
Chemical Shift Ref Cpd:TSP
Temperature:25C
Number Of Scans:128
Spectral Width:6000 Hz
Num Data Points Acquired:16K
Line Broadening:0.5 Hz
Binned Increment:0.00587
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