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MB Sample ID: SA013182
Local Sample ID: | H_H_plant4_26DAP |
Subject ID: | SU000310 |
Subject Type: | Plant |
Subject Species: | Zea mays |
Taxonomy ID: | 4577 |
Species Group: | Plant |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000310 |
Subject Type: | Plant |
Subject Species: | Zea mays |
Taxonomy ID: | 4577 |
Species Group: | Plant |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
H_H_plant4_26DAP | SA013182 | FL003114 | Both hot day and hot night | Temperature |
H_H_plant4_26DAP | SA013182 | FL003114 | 26 | Days after pollination |
Collection:
Collection ID: | CO000304 |
Collection Summary: | n/a |
Sample Type: | Maize kernel |
Collection Method: | manual removal from maize ear |
Collection Location: | University of Wisconsin Biotron |
Storage Conditions: | Frozen at -80C |
Treatment:
Treatment ID: | TR000324 |
Treatment Summary: | n/a |
Plant Growth Location: | Wisconsin Biotron Facility |
Plant Plot Design: | Random placement in growth facility |
Plant Light Period: | 16 h per day |
Plant Temp: | Normal day / normal night (28C day / 17C night; Hot day / normal night (38C / 17C); Hot day / hot night (38C / 28C) |
Plant Watering Regime: | Fully watered |
Plant Growth Stage: | Varied |
Plant Metab Quench Method: | Frozen at -80C |
Sample Preparation:
Sampleprep ID: | SP000318 |
Sampleprep Summary: | n/a |
Sampleprep Protocol Filename: | Maize_Tissue_sample_prep.pdf |
Combined analysis:
Analysis ID | AN000462 | AN000463 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Thermo Scientific-Dionex Ultimate 3000 | Thermo Scientific-Dionex Ultimate 3000 |
Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | Peak area | Peak area |
Chromatography:
Chromatography ID: | CH000329 |
Methods Filename: | Instrument_Parameters.pdf |
Instrument Name: | Thermo Scientific-Dionex Ultimate 3000 |
Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
Flow Rate: | 350 ul/min |
Internal Standard: | Appendix A - Internal Standard Prep GLCMS.pdf |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS000402 |
Analysis ID: | AN000462 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
Ion Mode: | POSITIVE |
Analysis Protocol File: | Instrument_Parameters.pdf |
MS ID: | MS000403 |
Analysis ID: | AN000463 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
Ion Mode: | NEGATIVE |
Analysis Protocol File: | Instrument_Parameters.pdf |