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MB Sample ID: SA013408
Local Sample ID: | B3P503 |
Subject ID: | SU000314 |
Subject Type: | Insect |
Subject Species: | Sarcophaga crassipalpis |
Taxonomy ID: | 59312 |
Genotype Strain: | Lab colony |
Age Or Age Range: | 15-25 day pupae |
Weight Or Weight Range: | 110-130 mg |
Animal Animal Supplier: | lab colony |
Animal Housing: | sterilite containers |
Animal Light Cycle: | 10:14 L:D |
Animal Feed: | beef liver |
Animal Inclusion Criteria: | must be in diapause |
Species Group: | Insect |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000314 |
Subject Type: | Insect |
Subject Species: | Sarcophaga crassipalpis |
Taxonomy ID: | 59312 |
Genotype Strain: | Lab colony |
Age Or Age Range: | 15-25 day pupae |
Weight Or Weight Range: | 110-130 mg |
Animal Animal Supplier: | lab colony |
Animal Housing: | sterilite containers |
Animal Light Cycle: | 10:14 L:D |
Animal Feed: | beef liver |
Animal Inclusion Criteria: | must be in diapause |
Species Group: | Insect |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
B3P503 | SA013408 | FL003139 | EN | Phase |
Collection:
Collection ID: | CO000308 |
Collection Summary: | Pupae were sampled at the appropriate time point and flash frozen in liquid nitrogen. Pupae were stored at -80C until extraction. |
Collection Protocol Comments: | Samples were homogenized in ice cold 80% methanol and centrifuged. Supernatant was retained and stored at -80C until analysis. |
Sample Type: | whole fly |
Collection Method: | flash freezing in liquid nitrogen |
Collection Location: | Hahn Lab, 3137 Steinmetz |
Collection Frequency: | Daily |
Collection Time: | see treatments |
Volumeoramount Collected: | one pupa per sample |
Storage Conditions: | -80C |
Collection Vials: | 1.5 ml microcentrifuge tubes |
Storage Vials: | 1.5 ml microcentrifuge tubes |
Collection Tube Temp: | room temperature |
Treatment:
Treatment ID: | TR000328 |
Treatment Summary: | ED=Early metabolic depression (48h) LD=Late metabolic depression (144h) AD=Arousing from metabolic depression (6h) IBA=Interbout arousal (15h) EN=Entering metabolic depression (30h) |
Sample Preparation:
Sampleprep ID: | SP000322 |
Sampleprep Summary: | This sample set is FleshFly tissue. The samples were received dried and frozen. The samples were reconstituted in 1 mL 90:10 Water:Acetonitrile then diluted 10x. |
Combined analysis:
Analysis ID | AN000470 | AN000471 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Thermo Scientific-Dionex Ultimate 3000 | Thermo Scientific-Dionex Ultimate 3000 |
Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | Peak height | Peak height |
Chromatography:
Chromatography ID: | CH000334 |
Methods Filename: | Metabolomics_LCMSProtocol.pdf |
Instrument Name: | Thermo Scientific-Dionex Ultimate 3000 |
Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
Flow Rate: | 0.350mL/min-0.600mL/min |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS000410 |
Analysis ID: | AN000470 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
Ion Mode: | POSITIVE |
Analysis Protocol File: | 469_Metabolomics_LCMSProtocol.pdf |
MS ID: | MS000411 |
Analysis ID: | AN000471 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
Ion Mode: | NEGATIVE |
Analysis Protocol File: | 469_Metabolomics_LCMSProtocol.pdf |