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MB Sample ID: SA013827
| Local Sample ID: | FS_47L |
| Subject ID: | SU000325 |
| Subject Type: | Animal |
| Subject Species: | Sus scrofa |
| Taxonomy ID: | 9823 |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000325 |
| Subject Type: | Animal |
| Subject Species: | Sus scrofa |
| Taxonomy ID: | 9823 |
| Species Group: | Mammal |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| FS_47L | SA013827 | FL003221 | Severe & Diffuse | atherosclerosis |
| FS_47L | SA013827 | FL003221 | High fat and salt | diet |
| FS_47L | SA013827 | FL003221 | CH | phenotype |
| FS_47L | SA013827 | FL003221 | female | sex |
Collection:
| Collection ID: | CO000319 |
| Collection Summary: | - |
| Sample Type: | Serum |
| Storage Conditions: | -80C |
Treatment:
| Treatment ID: | TR000339 |
| Treatment Summary: | - |
Sample Preparation:
| Sampleprep ID: | SP000333 |
| Sampleprep Summary: | Serum samples were transferred to labeled tubes. A total of 112 study samples were thawed on ice for sample preparation 350 uL of serum sample were thawed and transferred to labeled tubes on ice where they were prepared and eventually mixed with 700uL master mix containing 630 uL of phosphate buffer and 70 uL of Chenomx ISTD (5mM DSS, 6mM Imidazole, and 0.02% NaN3). Analytical quality control (QC) phenotypic pooled samples were generated by transferring a 75 µL of each sample of each respective phenotypical experimental sample into different 15 mL tubes. Whole study (total) pools were generated by transferring 200 uL of serum from each Pool sample into a 15 mL tube. The tubes were vortexed for 4 min on a multi-tube vortexer and centrifuged at 16,000 rcf for 4 min. A 600 µl aliquot of the supernatant was transferred into pre-labeled 5mm (4 inch) NMR tubes for data acquisition on a 700 MHz spectrometer.. |
| Sampleprep Protocol Filename: | Pig_Athersclerosis_Model_Procedures.docx |
Analysis:
| MB Sample ID: | SA013827 |
| Analysis ID: | AN000484 |
| Analysis Type: | NMR |
| Num Factors: | 15 |
NMR:
| NMR ID: | NM000060 |
| Analysis ID: | AN000484 |
| Instrument Name: | Bruker |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | Other |
| Field Frequency Lock: | Deuterium |
| Standard Concentration: | 0.5 mM |
| Spectrometer Frequency: | 700 MHz |
| NMR Probe: | 5 mm ATMA Cryoprobe |
| NMR Solvent: | D2O |
| NMR Tube Size: | 5 mm |
| Shimming Method: | Topshim |
| Pulse Sequence: | noesypr1d |
| Water Suppression: | yes |
| Receiver Gain: | 4.5 |
| Offset Frequency: | 3299.5 |
| Chemical Shift Ref Cpd: | DSS |
| Temperature: | 298.1 K |
| Number Of Scans: | 128 |
| Dummy Scans: | 4 |
| Acquisition Time: | 3.893 |
| Spectral Width: | 12.0227 ppm, 8.417 Hz |
| Num Data Points Acquired: | 65536 |
| Real Data Points: | 65536 |
| Line Broadening: | 0.5 Hz |
| Zero Filling: | yes |
| Apodization: | Lorentzian |
| Baseline Correction Method: | Polynomial |
| Chemical Shift Ref Std: | DSS-D6 |
| Binned Increment: | 0.04 |
| Binned Data Excluded Range: | water (4.68 – 5.2 ppm) methanol (3.33 - 3.36 ppm) imidazole (7.27 - 7.40 ppm) |
