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MB Sample ID: SA013892
| Local Sample ID: | DP2S125 |
| Subject ID: | SU000326 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | male/female |
| Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000326 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | male/female |
| Species Group: | Human |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| DP2S125 | SA013892 | FL003225 | Elderly No CAD | CAD |
Collection:
| Collection ID: | CO000320 |
| Collection Summary: | - |
| Sample Type: | plasma |
| Storage Conditions: | -80C |
Treatment:
| Treatment ID: | TR000340 |
| Treatment Summary: | - |
Sample Preparation:
| Sampleprep ID: | SP000334 |
| Sampleprep Summary: | Plasma samples were transferred to labeled tubes. A total of 219 study samples were thawed on ice for sample preparation200 uL of plasma sample were thawed and transferred to labeled tubes on ice where they were mixed with 50 uL Saline master mix (5mM Formate). Analytical quality control (QC) phenotypic pooled samples were generated by transferring a 25 µL of each sample of each respective phenotypical experimental sample into different 1.5 mL tubes. Whole study (total) pools were generated by transferring 200 uL of plasma from each Pool sample into a 2.0 mL tube. The tubes were vortexed for 4 min on a multi-tube vortexer and centrifuged at 16,000 rcf for 4 min. A 200 µl aliquot of the supernatant was transferred into pre-labeled 3mm NMR tubes for data acquisition on a 700 MHz spectrometer. |
| Sampleprep Protocol Filename: | CAD_Metabolomics_Procedures.docx |
Analysis:
| MB Sample ID: | SA013892 |
| Analysis ID: | AN000485 |
| Analysis Type: | NMR |
| Num Factors: | 6 |
NMR:
| NMR ID: | NM000061 |
| Analysis ID: | AN000485 |
| Instrument Name: | Bruker |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | Other |
| Field Frequency Lock: | Deuterium |
| Standard Concentration: | 0.5 mM |
| Spectrometer Frequency: | 700 MHz |
| NMR Probe: | 5 mm ATMA Cryoprobe |
| NMR Solvent: | D2O |
| NMR Tube Size: | 5 mm |
| Shimming Method: | Topshim |
| Pulse Sequence: | noesypr1d |
| Water Suppression: | yes |
| Receiver Gain: | 4.5 |
| Offset Frequency: | 3299.5 |
| Chemical Shift Ref Cpd: | DSS |
| Temperature: | 298.1 K |
| Number Of Scans: | 128 |
| Dummy Scans: | 4 |
| Acquisition Time: | 3.893 |
| Spectral Width: | 12.0227 ppm, 8.417 Hz |
| Num Data Points Acquired: | 65536 |
| Real Data Points: | 65536 |
| Line Broadening: | 0.5 Hz |
| Zero Filling: | yes |
| Apodization: | Lorentzian |
| Baseline Correction Method: | Polynomial |
| Chemical Shift Ref Std: | DSS-D6 |
| Binned Data Excluded Range: | water (4.60 - 5.10 ppm) EDTA (2.53 - 2.59 ppm, 2.67 -2.72 ppm, 3.06 - 3.15 ppm, 3.22 - 3.28 and 3.56 - 3.65ppm) noise regions (5.50-5.70 ppm, 5.90-6.80 ppm, 7.85-10.786 ppm) |
