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MB Sample ID: SA016349
Local Sample ID: | S_38 |
Subject ID: | SU000385 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000385 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Species Group: | Human |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
S_38 | SA016349 | FL003633 | - | Pool |
S_38 | SA016349 | FL003633 | Control | Metal Exposure |
S_38 | SA016349 | FL003633 | Male | Gender |
S_38 | SA016349 | FL003633 | - | Prenatal Smoking |
S_38 | SA016349 | FL003633 | Hispanic | Race |
S_38 | SA016349 | FL003633 | - | Asthma |
Collection:
Collection ID: | CO000379 |
Collection Summary: | Cord Blood Plasma |
Sample Type: | Blood |
Treatment:
Treatment ID: | TR000399 |
Treatment Summary: | NA |
Sample Preparation:
Sampleprep ID: | SP000392 |
Sampleprep Summary: | An aliquot (125 μl) of thawed plasma from each study sample was mixed with 375 μl of methanol, vortexed, and centrifuged. 400 uL of the supernatant was dried in vacuum overnight. Each sample was reconstituted with 250 μL of 0.2 mM phosphate buffer (pH 7.5) containing 10% Chenomx Internal Standard - 0.5 mM DSS-d6. Two random study pools were created by mixing 30 μl of plasma from a subset of study samples. Five replicates were created of each pool for a total of ten pooled samples, and these prepared identically to the study samples. The tubes were vortexed for 2 min on a multi-tube vortexer and centrifuged at 16,000 rcf for 10 min. A 200 µl aliquot of the supernatant was transferred into pre-labeled 3 mm (4”) NMR tubes for data acquisition on a 700 MHz spectrometer. |
Analysis:
MB Sample ID: | SA016349 |
Analysis ID: | AN000597 |
Analysis Type: | NMR |
Num Factors: | 78 |
NMR:
NMR ID: | NM000064 |
Analysis ID: | AN000597 |
Instrument Name: | Bruker Avance III |
Instrument Type: | FT-NMR |
NMR Experiment Type: | 1D 1H |
Spectrometer Frequency: | 700 MHz |