Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA016393

Local Sample ID:	S_35
Subject ID:	SU000385
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Species Group:	Human

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Subject:

Subject ID:	SU000385
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Species Group:	Human

Factors:

Local Sample ID	MB Sample ID	Factor Level ID	Level Value	Factor Name
S_35	SA016393	FL003665	Pool 1 Sample	Pool
S_35	SA016393	FL003665	high Cd & Pb = Cd 80th pct+, Pb 80th pct+	Metal Exposure
S_35	SA016393	FL003665	Female	Gender
S_35	SA016393	FL003665	1	Prenatal Smoking
S_35	SA016393	FL003665	Black	Race
S_35	SA016393	FL003665	-	Asthma

Collection:

Collection ID:	CO000379
Collection Summary:	Cord Blood Plasma
Sample Type:	Blood

Treatment:

Treatment ID:	TR000399
Treatment Summary:	NA

Sample Preparation:

Sampleprep ID:	SP000392
Sampleprep Summary:	An aliquot (125 μl) of thawed plasma from each study sample was mixed with 375 μl of methanol, vortexed, and centrifuged. 400 uL of the supernatant was dried in vacuum overnight. Each sample was reconstituted with 250 μL of 0.2 mM phosphate buffer (pH 7.5) containing 10% Chenomx Internal Standard - 0.5 mM DSS-d6. Two random study pools were created by mixing 30 μl of plasma from a subset of study samples. Five replicates were created of each pool for a total of ten pooled samples, and these prepared identically to the study samples. The tubes were vortexed for 2 min on a multi-tube vortexer and centrifuged at 16,000 rcf for 10 min. A 200 µl aliquot of the supernatant was transferred into pre-labeled 3 mm (4”) NMR tubes for data acquisition on a 700 MHz spectrometer.

Sampleprep ID:

SP000392

Sampleprep Summary:

An aliquot (125 μl) of thawed plasma from each study sample was mixed with 375 μl of methanol, vortexed, and centrifuged. 400 uL of the supernatant was dried in vacuum overnight. Each sample was reconstituted with 250 μL of 0.2 mM phosphate buffer (pH 7.5) containing 10% Chenomx Internal Standard - 0.5 mM DSS-d6. Two random study pools were created by mixing 30 μl of plasma from a subset of study samples. Five replicates were created of each pool for a total of ten pooled samples, and these prepared identically to the study samples. The tubes were vortexed for 2 min on a multi-tube vortexer and centrifuged at 16,000 rcf for 10 min. A 200 µl aliquot of the supernatant was transferred into pre-labeled 3 mm (4”) NMR tubes for data acquisition on a 700 MHz spectrometer.

Analysis:

MB Sample ID:	SA016393
Analysis ID:	AN000597
Analysis Type:	NMR
Num Factors:	78

NMR:

NMR ID:	NM000064
Analysis ID:	AN000597
Instrument Name:	Bruker Avance III
Instrument Type:	FT-NMR
NMR Experiment Type:	1D 1H
Spectrometer Frequency:	700 MHz