Return to study ST000366 main page
MB Sample ID: SA016496
| Local Sample ID: | CC0899 |
| Subject ID: | SU000387 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Male and Female |
| Human Race: | African American |
| Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000387 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Male and Female |
| Human Race: | African American |
| Species Group: | Human |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| CC0899 | SA016496 | FL003712 | Adenoma | Condition |
Collection:
| Collection ID: | CO000381 |
| Collection Summary: | None |
| Sample Type: | Colon |
| Storage Conditions: | -70C |
Treatment:
| Treatment ID: | TR000401 |
| Treatment Summary: | No treatment |
Sample Preparation:
| Sampleprep ID: | SP000394 |
| Sampleprep Summary: | Fecal samples (20 mg) were mixed with 1000 μL of D2O, mixed thoroughly by vortexing, and then centrifuged at 16000 x g for 20 min. The supernatants were removed and filtered through 0.22μ centrifuge filters at 16000 x g for 20 minutes. 540 μl of the filtrate was mixed with 60 μl of Chenomix Internal Standard mixture (containing DSS, Imidazole, and NaN3 in D2O). Aliquots of 550 μl were then transferred into 5mm NMR tubes. |
Analysis:
| MB Sample ID: | SA016496 |
| Analysis ID: | AN000599 |
| Analysis Type: | NMR |
| Num Factors: | 2 |
NMR:
| NMR ID: | NM000066 |
| Analysis ID: | AN000599 |
| Instrument Name: | Bruker950 MHz |
| Instrument Type: | Other |
| NMR Experiment Type: | Other |
| NMR Comments: | nmr experiment type: noesypr1d |
| Field Frequency Lock: | Deuterium |
| Standard Concentration: | 0.5 mM |
| Spectrometer Frequency: | 950 MHz |
| NMR Probe: | 5 mm ATMA Cryoprobe |
| NMR Solvent: | D2O |
| NMR Tube Size: | 5mm, 7 inch |
| Shimming Method: | Topshim |
| Pulse Sequence: | noesypr1d |
| Water Suppression: | yes |
| Pulse Width: | 8.44 us |
| Power Level: | 12.589 W |
| Receiver Gain: | 4 |
| Offset Frequency: | 4471.1 Hz |
| Presaturation Power Level: | 0.00003588 W |
| Chemical Shift Ref Cpd: | DSS |
| Temperature: | 298.1 K |
| Number Of Scans: | 256 |
| Dummy Scans: | 4 |
| Acquisition Time: | 1.075 s |
| Relaxation Delay: | 2 s |
| Spectral Width: | 16 ppm, 15.243 kHz |
| Num Data Points Acquired: | 32k |
| Real Data Points: | 32k |
| Line Broadening: | 0.5 Hz |
| Zero Filling: | yes |
| Apodization: | Lorentzian |
| Baseline Correction Method: | Polynomial |
| Chemical Shift Ref Std: | DSS |
| Binned Increment: | 0.04 |
| Binned Data Excluded Range: | 4.68 - 4.88 (water) |
