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MB Sample ID: SA017321
Local Sample ID: | 140813amssa10_1 |
Subject ID: | SU000402 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000402 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Species Group: | Human |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
140813amssa10_1 | SA017321 | FL003916 | INHA | Factor1 |
140813amssa10_1 | SA017321 | FL003916 | Matched control-C 6 | Factor2 |
140813amssa10_1 | SA017321 | FL003916 | n/a | Factor3 |
Collection:
Collection ID: | CO000396 |
Collection Summary: | All urine specimens were collected in the morning by clean catch methods. Samples were centrifuged at 4◦C to remove cell debris, and supernatants were aliquots of 1ml/tube, before storage at −80◦C until further analysis. |
Sample Type: | Urine |
Treatment:
Treatment ID: | TR000416 |
Treatment Summary: | Matched control urine vs. IC/PBS Urine |
Sample Preparation:
Sampleprep ID: | SP000409 |
Sampleprep Summary: | 1. Extraction with cold Methanol; extracts generated using protocols for GC profiling (“GC backups” re-analyzed by LC-MS). 2. Samples are taken to dryness and stored at -20 degree C until analysis. |
Sampleprep Protocol Filename: | SP_RP18_Protocol_KimUrine_IG102814.docx |
Combined analysis:
Analysis ID | AN000615 |
---|---|
Analysis type | MS |
Chromatography type | GC |
Chromatography system | Agilent 6890N |
Column | Restek Corporation Rtx-5Sil MS |
MS Type | EI |
MS instrument type | GC-TOF |
MS instrument name | Leco Pegasus III GC TOF |
Ion Mode | POSITIVE |
Units | counts |
Chromatography:
Chromatography ID: | CH000441 |
Methods Filename: | Data_Dictionary_Fiehn_laboratory_GCTOF_MS_primary_metabolism_10-15-2013_general.pdf |
Instrument Name: | Agilent 6890N |
Column Name: | Restek Corporation Rtx-5Sil MS |
Column Pressure: | 7.7 PSI |
Column Temperature: | 50-330C |
Flow Rate: | 1 ml/min |
Injection Temperature: | 50 C ramped to 250 C by 12 C/s |
Sample Injection: | 0.5 uL |
Oven Temperature: | 50°C for 1 min, then ramped at 20°C/min to 330°C, held constant for 5 min |
Transferline Temperature: | 230C |
Washing Buffer: | Ethyl Acetate |
Sample Loop Size: | 30 m length x 0.25 mm internal diameter |
Randomization Order: | Excel generated |
Chromatography Type: | GC |
MS:
MS ID: | MS000548 |
Analysis ID: | AN000615 |
Instrument Name: | Leco Pegasus III GC TOF |
Instrument Type: | GC-TOF |
MS Type: | EI |
Ion Mode: | POSITIVE |
Ion Source Temperature: | 250 C |
Ionization Energy: | 70 eV |
Mass Accuracy: | Nominal |
Source Temperature: | 250 C |
Scan Range Moverz: | 85-500 Da |
Scanning Cycle: | 17 Hz |
Scanning Range: | 85-500 Da |
Skimmer Voltage: | 1850 V |