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MB Sample ID: SA017336
| Local Sample ID: | Inj050_SA097_KimUrine.d |
| Subject ID: | SU000403 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000403 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| Inj050_SA097_KimUrine.d | SA017336 | FL003920 | CSMC | Cohort |
| Inj050_SA097_KimUrine.d | SA017336 | FL003920 | IC/PBS Urine | Treatment |
| Inj050_SA097_KimUrine.d | SA017336 | FL003920 | SEVERE | SubCondition |
Collection:
| Collection ID: | CO000397 |
| Collection Summary: | All urine specimens were collected in the morning by clean catch methods. Samples were centrifuged at 4◦C to remove cell debris, and supernatants were aliquots of 1ml/tube, before storage at −80◦C until further analysis. |
| Sample Type: | Urine |
Treatment:
| Treatment ID: | TR000417 |
| Treatment Summary: | Matched control urine vs. IC/PBS Urine |
Sample Preparation:
| Sampleprep ID: | SP000410 |
| Sampleprep Summary: | 1. The dry sample is resuspended in 50 uL of Water/acetonitrile 95:5 containing 100 ng/mL of CUDA (12-[[(cyclohexylamino)carbonyl]amino]-dodecanoic acid) as internal standard, vortexed for 10 sec, sonicated for 5 minutes, then centrifuged for 3 min at 14,000 rcf. 2. The samples are transferred to amber vials with micro-inserts and capped immediately, and stored at 4 degree C until and during analysis |
| Sampleprep Protocol Filename: | SP_RP18_Protocol_KimUrine_IG102814.docx |
Combined analysis:
| Analysis ID | AN000616 | AN000617 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Agilent 1290 Infinity | Agilent 1290 Infinity |
| Column | Waters Acquity BEH C18 (100 x 2.1mm,1.7um) | Waters Acquity BEH C18 (100 x 2.1mm,1.7um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Agilent 6530 QTOF | Agilent 6530 QTOF |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | counts | counts |
Chromatography:
| Chromatography ID: | CH000442 |
| Instrument Name: | Agilent 1290 Infinity |
| Column Name: | Waters Acquity BEH C18 (100 x 2.1mm,1.7um) |
| Column Pressure: | 1200 bar |
| Column Temperature: | 65 C |
| Flow Gradient: | 0% B to 99%B |
| Flow Rate: | 0.5 mL/min |
| Internal Standard: | CUDA |
| Sample Injection: | 3uL |
| Solvent A: | 95% water/5% acetonitrile; 0.1% acetic acid |
| Solvent B: | 100% acetonitrile; 0.1% acetic acid |
| Analytical Time: | 15 min |
| Target Sample Temperature: | Autosampler temp 4 C |
| Randomization Order: | Excel generated |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000549 |
| Analysis ID: | AN000616 |
| Instrument Name: | Agilent 6530 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Capillary Voltage: | 3500 |
| Collision Gas: | Nitrogen |
| Dry Gas Flow: | 8 L/min |
| Dry Gas Temp: | 325 C |
| Fragment Voltage: | 120 |
| Fragmentation Method: | Auto MS/MS |
| Ion Source Temperature: | 325 C |
| Ion Spray Voltage: | 1000 |
| Ionization: | Pos |
| Precursor Type: | Intact Molecule |
| Reagent Gas: | Nitrogen |
| Source Temperature: | 325 C |
| Dataformat: | .d |
| Desolvation Gas Flow: | 11 L/min |
| Desolvation Temperature: | 350 C |
| Nebulizer: | 35 psig |
| Octpole Voltage: | 750 |
| Resolution Setting: | extended dynamic range |
| Scan Range Moverz: | 60-1700 Da |
| Scanning Cycle: | 2 Hz |
| Scanning Range: | 60-1700 Da |
| Skimmer Voltage: | 65 |
| MS ID: | MS000550 |
| Analysis ID: | AN000617 |
| Instrument Name: | Agilent 6530 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Capillary Voltage: | 3500 |
| Collision Gas: | Nitrogen |
| Dry Gas Flow: | 13 L/min |
| Dry Gas Temp: | 200 C |
| Fragment Voltage: | 175 |
| Fragmentation Method: | Auto MS/MS |
| Ion Source Temperature: | 325 C |
| Ion Spray Voltage: | 1000 |
| Ionization: | Neg |
| Dataformat: | .d |
| Desolvation Gas Flow: | 11 L/min |
| Desolvation Temperature: | 350 C |
| Nebulizer: | 35 psig |
| Octpole Voltage: | 750 |
| Scan Range Moverz: | 60-1700 Da |
| Scanning Cycle: | 2Hz |
| Scanning Range: | 60-1700 |
| Skimmer Voltage: | 65 |
