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MB Sample ID: SA017409
Local Sample ID: | Inj046_SA024_KimUrine.d |
Subject ID: | SU000403 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000403 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Species Group: | Human |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
Inj046_SA024_KimUrine.d | SA017409 | FL003925 | INHA | Cohort |
Inj046_SA024_KimUrine.d | SA017409 | FL003925 | Matched Control Urine | Treatment |
Inj046_SA024_KimUrine.d | SA017409 | FL003925 | n/a | SubCondition |
Collection:
Collection ID: | CO000397 |
Collection Summary: | All urine specimens were collected in the morning by clean catch methods. Samples were centrifuged at 4◦C to remove cell debris, and supernatants were aliquots of 1ml/tube, before storage at −80◦C until further analysis. |
Sample Type: | Urine |
Treatment:
Treatment ID: | TR000417 |
Treatment Summary: | Matched control urine vs. IC/PBS Urine |
Sample Preparation:
Sampleprep ID: | SP000410 |
Sampleprep Summary: | 1. The dry sample is resuspended in 50 uL of Water/acetonitrile 95:5 containing 100 ng/mL of CUDA (12-[[(cyclohexylamino)carbonyl]amino]-dodecanoic acid) as internal standard, vortexed for 10 sec, sonicated for 5 minutes, then centrifuged for 3 min at 14,000 rcf. 2. The samples are transferred to amber vials with micro-inserts and capped immediately, and stored at 4 degree C until and during analysis |
Sampleprep Protocol Filename: | SP_RP18_Protocol_KimUrine_IG102814.docx |
Combined analysis:
Analysis ID | AN000616 | AN000617 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Agilent 1290 Infinity | Agilent 1290 Infinity |
Column | Waters Acquity BEH C18 (100 x 2.1mm,1.7um) | Waters Acquity BEH C18 (100 x 2.1mm,1.7um) |
MS Type | ESI | ESI |
MS instrument type | QTOF | QTOF |
MS instrument name | Agilent 6530 QTOF | Agilent 6530 QTOF |
Ion Mode | POSITIVE | NEGATIVE |
Units | counts | counts |
Chromatography:
Chromatography ID: | CH000442 |
Instrument Name: | Agilent 1290 Infinity |
Column Name: | Waters Acquity BEH C18 (100 x 2.1mm,1.7um) |
Column Pressure: | 1200 bar |
Column Temperature: | 65 C |
Flow Gradient: | 0% B to 99%B |
Flow Rate: | 0.5 mL/min |
Internal Standard: | CUDA |
Sample Injection: | 3uL |
Solvent A: | 95% water/5% acetonitrile; 0.1% acetic acid |
Solvent B: | 100% acetonitrile; 0.1% acetic acid |
Analytical Time: | 15 min |
Target Sample Temperature: | Autosampler temp 4 C |
Randomization Order: | Excel generated |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS000549 |
Analysis ID: | AN000616 |
Instrument Name: | Agilent 6530 QTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
Ion Mode: | POSITIVE |
Capillary Voltage: | 3500 |
Collision Gas: | Nitrogen |
Dry Gas Flow: | 8 L/min |
Dry Gas Temp: | 325 C |
Fragment Voltage: | 120 |
Fragmentation Method: | Auto MS/MS |
Ion Source Temperature: | 325 C |
Ion Spray Voltage: | 1000 |
Ionization: | Pos |
Precursor Type: | Intact Molecule |
Reagent Gas: | Nitrogen |
Source Temperature: | 325 C |
Dataformat: | .d |
Desolvation Gas Flow: | 11 L/min |
Desolvation Temperature: | 350 C |
Nebulizer: | 35 psig |
Octpole Voltage: | 750 |
Resolution Setting: | extended dynamic range |
Scan Range Moverz: | 60-1700 Da |
Scanning Cycle: | 2 Hz |
Scanning Range: | 60-1700 Da |
Skimmer Voltage: | 65 |
MS ID: | MS000550 |
Analysis ID: | AN000617 |
Instrument Name: | Agilent 6530 QTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
Ion Mode: | NEGATIVE |
Capillary Voltage: | 3500 |
Collision Gas: | Nitrogen |
Dry Gas Flow: | 13 L/min |
Dry Gas Temp: | 200 C |
Fragment Voltage: | 175 |
Fragmentation Method: | Auto MS/MS |
Ion Source Temperature: | 325 C |
Ion Spray Voltage: | 1000 |
Ionization: | Neg |
Dataformat: | .d |
Desolvation Gas Flow: | 11 L/min |
Desolvation Temperature: | 350 C |
Nebulizer: | 35 psig |
Octpole Voltage: | 750 |
Scan Range Moverz: | 60-1700 Da |
Scanning Cycle: | 2Hz |
Scanning Range: | 60-1700 |
Skimmer Voltage: | 65 |