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MB Sample ID: SA017925
| Local Sample ID: | Sarz164_51762post_247_CSHMS.d |
| Subject ID: | SU000408 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000408 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| Sarz164_51762post_247_CSHMS.d | SA017925 | FL003975 | Post-Exercise | Treatment |
Collection:
| Collection ID: | CO000402 |
| Collection Summary: | Whole blood was processed into serum and plasma and stored at 80C |
| Collection Protocol Filename: | StudyDesign-MarkSarzynski-72315.pdf |
| Sample Type: | Blood |
| Blood Serum Or Plasma: | Plasma |
Treatment:
| Treatment ID: | TR000422 |
| Treatment Summary: | Before and after 20 weeks of endurance exercise training: samples were taken at baseline (N=125) and postexercise training (N=125) in the same individuals |
| Treatment Protocol Filename: | StudyDesign-MarkSarzynski-72315.pdf |
Sample Preparation:
| Sampleprep ID: | SP000415 |
| Sampleprep Summary: | 1) Degas both “MeOH with QCmix” and MTBE with Cholesterol Ester 22:1 bysonication 2) Store solvents in the –20°C freezer to pre-chill 3) Thaw each 20 μL plasma aliquot at room temperature. Once thawed (~10min) place liquid plasma samples on ice. 4) Add 225 μL cold “MeOH with QC mix” (see SOP “QC mix for LC-MS lipid analysis”). Keep MeOH on ice during extraction 5) Vortex each sample for 10s, keeping the rest on ice during all the extraction. 6) Add 750 μL of cold MTBE with 22:1 CE, keep MTBE on ice during extraction Vortex for 10s 7) Shake for 6min at 4°C in the orbital mixer. Add 188 μL room temperature LC/MS grade water. 8)Vortex for 20 s 9) Centrifuge for 2 min @ 14,000 rcf (12300 rpm) 10) Remove supernatant, splitting into two aliquots of 350 μL, keeping one at –20°C for backup 11) Dry samples to complete dryness in the speed vacuum concentration system |
| Sampleprep Protocol Filename: | SOP_Lipid_Extraction_Plasma_06242013 (1).pdf |
| Processing Method: | Homogenization |
Combined analysis:
| Analysis ID | AN000622 | AN000623 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Agilent 6530 | Agilent 6550 |
| Column | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Agilent 6530 QTOF | Agilent 6550 QTOF |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | counts | counts |
Chromatography:
| Chromatography ID: | CH000447 |
| Methods Filename: | Data_Dictionary_Fiehn_laboratory_CSH_QTOF_lipidomics_05-29-2014.pdf |
| Instrument Name: | Agilent 6530 |
| Column Name: | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) |
| Column Pressure: | 450-850 bar |
| Column Temperature: | 65 C |
| Flow Gradient: | 15% B to 99%B |
| Flow Rate: | 0.6 mL/min |
| Internal Standard: | See data dictionary |
| Retention Time: | See data dictionary |
| Sample Injection: | 1.67 uL |
| Solvent A: | 60% acetonitrile/40% water; 10mM formic acid; 10mM ammonium formate |
| Solvent B: | 90% isopropanol/10% acetonitrile; 10mM formic acid; 10mM ammonium formate |
| Analytical Time: | 13 min |
| Capillary Voltage: | 3500 V |
| Time Program: | 15 min |
| Weak Wash Solvent Name: | Isopropanol |
| Strong Wash Solvent Name: | Isopropanol |
| Target Sample Temperature: | Autosampler temp 4 C |
| Randomization Order: | Excel generated |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH000448 |
| Methods Filename: | Data_Dictionary_Fiehn_laboratory_CSH_QTOF_lipidomics_05-29-2014.pdf |
| Instrument Name: | Agilent 6550 |
| Column Name: | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) |
| Column Pressure: | 450-850 bar |
| Column Temperature: | 65 C |
| Flow Gradient: | 15% B to 99%B |
| Flow Rate: | 0.6 mL/min |
| Internal Standard: | see data dictionary |
| Retention Time: | see data dictionary |
| Sample Injection: | 5 uL |
| Solvent A: | 95% water/5% acetonitrile; 0.1% acetic acid |
| Solvent B: | 100% acetonitrile; 0.1% acetic acid |
| Analytical Time: | 13 min |
| Capillary Voltage: | 3500 V |
| Time Program: | 15 min |
| Weak Wash Solvent Name: | Isopropanol |
| Strong Wash Solvent Name: | Isopropanol |
| Target Sample Temperature: | Autosampler temp 4 C |
| Randomization Order: | Excel generated |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000555 |
| Analysis ID: | AN000622 |
| Instrument Name: | Agilent 6530 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Capillary Voltage: | 3500 |
| Collision Gas: | Nitrogen |
| Dry Gas Flow: | 8 L/min |
| Dry Gas Temp: | 325 C |
| Fragment Voltage: | 120 V |
| Fragmentation Method: | Auto MS/MS |
| Ion Source Temperature: | 325 C |
| Ion Spray Voltage: | 1000 V |
| Ionization: | Pos |
| Precursor Type: | Intact Molecule |
| Reagent Gas: | Nitrogen |
| Source Temperature: | 325 C |
| Dataformat: | .d |
| Desolvation Gas Flow: | 11 L/min |
| Desolvation Temperature: | 350 C |
| Nebulizer: | 35 psig |
| Octpole Voltage: | 750 V |
| Resolution Setting: | extended dynamic range |
| Scan Range Moverz: | 60-1700 Da |
| Scanning Cycle: | 2 Hz |
| Scanning Range: | 60-1700 Da |
| Skimmer Voltage: | 65 V |
| MS ID: | MS000556 |
| Analysis ID: | AN000623 |
| Instrument Name: | Agilent 6550 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Capillary Voltage: | 3500 |
| Collision Gas: | Nitrogen |
| Dry Gas Flow: | 13 L/min |
| Dry Gas Temp: | 200 C |
| Fragment Voltage: | 175 V |
| Fragmentation Method: | Auto MS/MS |
| Ion Source Temperature: | 325 C |
| Ion Spray Voltage: | 1000 |
| Ionization: | Neg |
| Dataformat: | .d |
| Desolvation Gas Flow: | 11 L/min |
| Desolvation Temperature: | 350 C |
| Nebulizer: | 35 psig |
| Octpole Voltage: | 750 V |
| Scan Range Moverz: | 60-1700 Da |
| Scanning Cycle: | 2Hz |
| Scanning Range: | 60-1700 |
| Skimmer Voltage: | 65 V |
