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MB Sample ID: SA019132
| Local Sample ID: | PU3T3_11_39gw_1_1 |
| Subject ID: | SU000419 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 18-41 years |
| Gender: | Female |
| Human Race: | Caucasian |
| Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000419 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 18-41 years |
| Gender: | Female |
| Human Race: | Caucasian |
| Species Group: | Human |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| PU3T3_11_39gw_1_1 | SA019132 | FL004690 | Control group 2 | group ID |
| PU3T3_11_39gw_1_1 | SA019132 | FL004690 | NA | Treatment duration |
Collection:
| Collection ID: | CO000413 |
| Collection Summary: | Urine samples were collected at routine medical appointments and diabetes counselling appointments, for independent groups of women in their 2nd and 3rd trimesters of pregnancy. Samples were collected under ethical approval of the Hospital Center of Coimbra (Refs.18/04 and 29/09) and informed consents were obtained from each woman. |
| Sample Type: | Urine |
| Collection Location: | Maternity Bissaya Barreto, Coimbra, Portugal |
| Collection Time: | Non-fasting, during morning |
| Volumeoramount Collected: | 50 mL |
| Storage Conditions: | -80ºC |
| Additives: | No |
Treatment:
| Treatment ID: | TR000433 |
| Treatment Summary: | Subjects were treated with diet or insulin |
| Treatment Compound: | Insulin |
Sample Preparation:
| Sampleprep ID: | SP000426 |
| Sampleprep Summary: | Before analysis, samples were thawed and 800 μL were centrifuged (4500g, 5 min). Then, 60 μL of 1.5 M phosphate buffer pH 7 in D2O, 0.1% Na+/3-trimethylsilyl-propionate (TSP) were added to 540 μL of supernatant, followed by pH readjustment to 7.00±0.02 with KOD (4 M) or DCl (4 M). The mixture was centrifuged (4500 g, 5 min) and 550 μL transferred to 5 mm NMR tubes. |
Analysis:
| MB Sample ID: | SA019132 |
| Analysis ID: | AN000635 |
| Laboratory Name: | CICECO metabolomics group |
| Analysis Type: | NMR |
| Analysis Protocol File: | noesypr1d |
| Software Version: | Topspin 3.2 |
| Num Factors: | 21 |
NMR:
| NMR ID: | NM000067 |
| Analysis ID: | AN000635 |
| Instrument Name: | Bruker Avance DRX 500 |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D 1H |
| NMR Comments: | Standard experiment (noesypr1d) |
| Field Frequency Lock: | Deuterium |
| Spectrometer Frequency: | 500 MHz |
| NMR Probe: | BBI |
| NMR Solvent: | D2O+H2O |
| NMR Tube Size: | 5 mm |
| Shimming Method: | Automatic |
| Pulse Sequence: | noesypr1d |
| Water Suppression: | presaturation |
| Receiver Gain: | 32 |
| Chemical Shift Ref Cpd: | TSP |
| Temperature: | 300 K |
| Number Of Scans: | 128 |
| Dummy Scans: | 4 |
| Acquisition Time: | 3.25 s |
| Relaxation Delay: | 32 |
| Spectral Width: | 20.16 ppm |
| Num Data Points Acquired: | 64 k |
| Real Data Points: | 64 k |
| Line Broadening: | 0.3 Hz |
| Baseline Correction Method: | manual |
| Chemical Shift Ref Std: | TSP (0.0 ppm) |
| Binned Increment: | full resolution |
| Binned Data Excluded Range: | 4.60-5.05 ppm |
