Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA019987

Local Sample ID:	F_15
Subject ID:	SU000430
Subject Type:	Mice
Subject Species:	Mus musculus
Taxonomy ID:	10090
Species Group:	Mammal

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Subject:

Subject ID:	SU000430
Subject Type:	Mice
Subject Species:	Mus musculus
Taxonomy ID:	10090
Species Group:	Mammal

Factors:

Local Sample ID	MB Sample ID	Factor Level ID	Level Value	Factor Name
F_15	SA019987	FL004760	OT	Treatment
F_15	SA019987	FL004760	S1	Time Point
F_15	SA019987	FL004760	N	Pool

Collection:

Collection ID:	CO000424
Collection Summary:	24 hr after the social test, mice were deeply anesthetized with 5% isoflurane, followed by rapid decapitation. Brains were removed, rinsed with ice-cold water, and then rapidly dissected into the following 5 regions: forebrain, midbrain, cerebellum, hindbrain, and olfactory bulbs. The dissected parts were flash-frozen on dry ice and stored at -80o C. Samples were coded by treatment: vehicle-treated mice (n=8) and OT-treated mice (n=12).
Sample Type:	Feces

Treatment:

Treatment ID:	TR000444
Treatment Summary:	BALB/cByJ male mice (4-5 weeks in age; Jackson Laboratory, Bar Harbor, ME) were given four treatments of either vehicle or OT (1.0 mg/kg; IP) across 8 days, with at least 48 hours between each injection. Mice were evaluated in a 3-chamber choice test for social preference 24 hr after the final injection.

Sample Preparation:

Sampleprep ID:	SP000437
Sampleprep Summary:	Fecal pellets were transferred into a pre-labeled MagnaLyser tube and massed. Phosphate buffer was added depending on the mass of each sample (500 uL for up to 100 mg and 1000 uL for more than 200 mg). Samples were homogenized using MagnaLyser for two 30 sec pulses and then centrifuged. The supernatant of each sample was transferred into pre-labeled 0.2 um nylon filter tube and centrifuged. A volume of each filtrate required to analyze 25 mg of sample was aliquoted, mixed with a volume of phosphate buffer to bring the total volume to 630 uL, and 70 uL of Chenomx ISTD. For a subset of samples with excess material, a total study pool was created by mixing the remaining volume. Five replicates were created, and these prepared identically to the study samples. The tubes were vortexed for 2 min on a multi-tube vortexer and centrifuged at 16,000 rcf for 4 min. A 600 µl aliquot of the supernatant was transferred into pre-labeled 5 mm (4”) NMR tubes for data acquisition on a 700 MHz spectrometer.

Sampleprep ID:

SP000437

Sampleprep Summary:

Fecal pellets were transferred into a pre-labeled MagnaLyser tube and massed. Phosphate buffer was added depending on the mass of each sample (500 uL for up to 100 mg and 1000 uL for more than 200 mg). Samples were homogenized using MagnaLyser for two 30 sec pulses and then centrifuged. The supernatant of each sample was transferred into pre-labeled 0.2 um nylon filter tube and centrifuged. A volume of each filtrate required to analyze 25 mg of sample was aliquoted, mixed with a volume of phosphate buffer to bring the total volume to 630 uL, and 70 uL of Chenomx ISTD. For a subset of samples with excess material, a total study pool was created by mixing the remaining volume. Five replicates were created, and these prepared identically to the study samples. The tubes were vortexed for 2 min on a multi-tube vortexer and centrifuged at 16,000 rcf for 4 min. A 600 µl aliquot of the supernatant was transferred into pre-labeled 5 mm (4”) NMR tubes for data acquisition on a 700 MHz spectrometer.

Analysis:

MB Sample ID:	SA019987
Analysis ID:	AN000649
Analysis Type:	NMR
Num Factors:	16

NMR:

NMR ID:	NM000071
Analysis ID:	AN000649
Instrument Name:	Bruker Avance III
Instrument Type:	FT-NMR
NMR Experiment Type:	1D 1H
Spectrometer Frequency:	700 MHz