Return to study ST000428 main page
MB Sample ID: SA021651
Local Sample ID: | A50 |
Subject ID: | SU000449 |
Subject Type: | Animal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000449 |
Subject Type: | Animal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Species Group: | Mammal |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
A50 | SA021651 | FL005312 | Sham 20mth | Sample |
Collection:
Collection ID: | CO000443 |
Collection Summary: | Gastrocnemius tissue was harvested and then flash frozen in a liquid nitrogen cooled biopress |
Sample Type: | Muscle |
Treatment:
Treatment ID: | TR000463 |
Treatment Summary: | Treatment Summary Fraction of gastrocnemius tissue weighed (25–50 mg wet weight), then the finely cut up tissue quickly added to fresh pre-made buffer (50 % acetyl-nitrile, 50 % water, 0.3% formic acid) at a standard concentration of 25 mg/475 mcl buffer then fully homogenized on ice for 10–25 s and placed on dry ice/stored at - 80C |
Sample Preparation:
Sampleprep ID: | SP000456 |
Sampleprep Summary: | Amino acids and acyl-carnitines were analyzed using stable isotope dilution techniques. Amino acids and acyl-carnitine measurements were made by flow injection tandem mass spectrometry |
Combined analysis:
Analysis ID | AN000678 |
---|---|
Analysis type | MS |
Chromatography type | GC |
Chromatography system | Waters Acquity |
Column | Waters ACQUITY UPLC (1.7um) |
MS Type | EI |
MS instrument type | Single quadrupole |
MS instrument name | Agilent 5975 |
Ion Mode | POSITIVE |
Units | uM |
Chromatography:
Chromatography ID: | CH000490 |
Instrument Name: | Waters Acquity |
Column Name: | Waters ACQUITY UPLC (1.7um) |
Chromatography Type: | GC |
MS:
MS ID: | MS000604 |
Analysis ID: | AN000678 |
Instrument Name: | Agilent 5975 |
Instrument Type: | Single quadrupole |
MS Type: | EI |
Ion Mode: | POSITIVE |