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MB Sample ID: SA022102
| Local Sample ID: | S_9 |
| Subject ID: | SU000460 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Human Race: | African American |
| Human Ethnicity: | African American |
| Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000460 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Human Race: | African American |
| Human Ethnicity: | African American |
| Species Group: | Human |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| S_9 | SA022102 | FL006450 | F | Gender |
| S_9 | SA022102 | FL006450 | WC | Group |
Collection:
| Collection ID: | CO000454 |
| Collection Summary: | Blood samples were collected from each subject using 10 ml blood BD Vacutainer tubes containing coagulants (EDTA). Tubes were centrifuged at 1850 x g for 10 min at room temperature and the upper layer serum in 1 ml aliquots was transferred to cryo-preservative tubes and preserved in –80 C freezer. |
| Sample Type: | Blood |
Treatment:
| Treatment ID: | TR000474 |
| Treatment Summary: | No treatments. |
Sample Preparation:
| Sampleprep ID: | SP000467 |
| Sampleprep Summary: | Aliquots of each de-identified sample were shipped to the NIH RTI-RCMRC on dry ice and immediately stored at -80 °C after being logged in for metabolomics analysis. A total of 66 study samples were thawed on ice for sample preparation. A 300 uL aliquot of plasma was transferred to new labeled tubes for each study sample. Analytical quality control (QC) phenotypic pooled samples were generated by transferring a 80µL aliquot of each sample from each respective phenotypic group (Control-women, BCa-women, Control-men and PCa-men) into different 1.5 mL tubes. Phenotypic pooled samples were vortexed and 300 uL aliquots were transferred into 3 tubes/group. A total study pool was generated by transferring 250 uL of plasma from each Phenotypic pooled sample into a new 1.5 mL tube. The Total Pool sample was vortexed and 300 uL aliquots were transferred into 3 Total Pool-labeled tubes. For extraction, 900 uL of MeOH was added to all tubes, they were vortexed for 2 min on a multi-tube vortexer and centrifuged at 16,000 rcf for 5 min. A 1000 µl aliquot of the supernatant was transferred into pre-labeled 2.0mL LoBind Eppendorf tubes, and samples were lyophilized to complete dryness overnight. Samples were reconstituted with 700 uL of NMR Master Mix solution containing Chenomx ISTD: DSS-d6 and Phosphate Buffer at 7.4 pH. The tubes were vortexed for 2 min on a multi-tube vortexer and centrifuged at 16,000 rcf for 5 min. A 600uL aliquot of supernatants were transferred into a pre-labeled 5mm 4 NMR tubes for data acquisition on a 700 MHz spectrometer. |
Analysis:
| MB Sample ID: | SA022102 |
| Analysis ID: | AN000690 |
| Analysis Type: | NMR |
| Num Factors: | 4 |
NMR:
| NMR ID: | NM000075 |
| Analysis ID: | AN000690 |
| Instrument Name: | Bruker |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D 1H |
| Field Frequency Lock: | Deuterium |
| Standard Concentration: | 0.5mM |
| Spectrometer Frequency: | 700 MHz |
| NMR Probe: | 5mm ATMA Cryoprobe |
| NMR Solvent: | D2O |
| NMR Tube Size: | 5mm |
| Shimming Method: | Topshim |
| Water Suppression: | yes |
| Receiver Gain: | 4.5 |
| Offset Frequency: | 3299 |
| Chemical Shift Ref Cpd: | DSS |
| Temperature: | 298.1K |
| Number Of Scans: | 128 |
| Dummy Scans: | 4 |
| Acquisition Time: | 3.893s |
| Relaxation Delay: | 2s |
| Spectral Width: | 12.0277ppm |
| Num Data Points Acquired: | 65536 |
| Real Data Points: | 65536 |
| Line Broadening: | 0.5Hz |
| Zero Filling: | yes |
| Apodization: | Lorentzian |
| Baseline Correction Method: | Polynomial |
| Chemical Shift Ref Std: | DSS-d6 |
| Binned Increment: | 0.04ppm |
