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MB Sample ID: SA022121
Local Sample ID: | S_21 |
Subject ID: | SU000460 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Human Race: | African American |
Human Ethnicity: | African American |
Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000460 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Human Race: | African American |
Human Ethnicity: | African American |
Species Group: | Human |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
S_21 | SA022121 | FL006451 | M | Gender |
S_21 | SA022121 | FL006451 | MC | Group |
Collection:
Collection ID: | CO000454 |
Collection Summary: | Blood samples were collected from each subject using 10 ml blood BD Vacutainer tubes containing coagulants (EDTA). Tubes were centrifuged at 1850 x g for 10 min at room temperature and the upper layer serum in 1 ml aliquots was transferred to cryo-preservative tubes and preserved in –80 C freezer. |
Sample Type: | Blood |
Treatment:
Treatment ID: | TR000474 |
Treatment Summary: | No treatments. |
Sample Preparation:
Sampleprep ID: | SP000467 |
Sampleprep Summary: | Aliquots of each de-identified sample were shipped to the NIH RTI-RCMRC on dry ice and immediately stored at -80 °C after being logged in for metabolomics analysis. A total of 66 study samples were thawed on ice for sample preparation. A 300 uL aliquot of plasma was transferred to new labeled tubes for each study sample. Analytical quality control (QC) phenotypic pooled samples were generated by transferring a 80µL aliquot of each sample from each respective phenotypic group (Control-women, BCa-women, Control-men and PCa-men) into different 1.5 mL tubes. Phenotypic pooled samples were vortexed and 300 uL aliquots were transferred into 3 tubes/group. A total study pool was generated by transferring 250 uL of plasma from each Phenotypic pooled sample into a new 1.5 mL tube. The Total Pool sample was vortexed and 300 uL aliquots were transferred into 3 Total Pool-labeled tubes. For extraction, 900 uL of MeOH was added to all tubes, they were vortexed for 2 min on a multi-tube vortexer and centrifuged at 16,000 rcf for 5 min. A 1000 µl aliquot of the supernatant was transferred into pre-labeled 2.0mL LoBind Eppendorf tubes, and samples were lyophilized to complete dryness overnight. Samples were reconstituted with 700 uL of NMR Master Mix solution containing Chenomx ISTD: DSS-d6 and Phosphate Buffer at 7.4 pH. The tubes were vortexed for 2 min on a multi-tube vortexer and centrifuged at 16,000 rcf for 5 min. A 600uL aliquot of supernatants were transferred into a pre-labeled 5mm 4 NMR tubes for data acquisition on a 700 MHz spectrometer. |
Analysis:
MB Sample ID: | SA022121 |
Analysis ID: | AN000690 |
Analysis Type: | NMR |
Num Factors: | 4 |
NMR:
NMR ID: | NM000075 |
Analysis ID: | AN000690 |
Instrument Name: | Bruker |
Instrument Type: | FT-NMR |
NMR Experiment Type: | 1D 1H |
Field Frequency Lock: | Deuterium |
Standard Concentration: | 0.5mM |
Spectrometer Frequency: | 700 MHz |
NMR Probe: | 5mm ATMA Cryoprobe |
NMR Solvent: | D2O |
NMR Tube Size: | 5mm |
Shimming Method: | Topshim |
Water Suppression: | yes |
Receiver Gain: | 4.5 |
Offset Frequency: | 3299 |
Chemical Shift Ref Cpd: | DSS |
Temperature: | 298.1K |
Number Of Scans: | 128 |
Dummy Scans: | 4 |
Acquisition Time: | 3.893s |
Relaxation Delay: | 2s |
Spectral Width: | 12.0277ppm |
Num Data Points Acquired: | 65536 |
Real Data Points: | 65536 |
Line Broadening: | 0.5Hz |
Zero Filling: | yes |
Apodization: | Lorentzian |
Baseline Correction Method: | Polynomial |
Chemical Shift Ref Std: | DSS-d6 |
Binned Increment: | 0.04ppm |