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MB Sample ID: SA022448
Local Sample ID: | MMV020548 |
Subject ID: | SU000462 |
Subject Type: | MACS purified infected RBCs |
Subject Species: | Plasmodium falciparum |
Taxonomy ID: | 5833 |
Species Group: | Microorganism |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000462 |
Subject Type: | MACS purified infected RBCs |
Subject Species: | Plasmodium falciparum |
Taxonomy ID: | 5833 |
Species Group: | Microorganism |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
MMV020548 | SA022448 | FL005453 | MMV020548 | Drug |
Collection:
Collection ID: | CO000456 |
Collection Summary: | Cells were pelleted and the media aspirated prior to methanol extraction |
Sample Type: | Plasmodium cells |
Treatment:
Treatment ID: | TR000476 |
Treatment Summary: | Magnetically purified iRBCs (~20uL) were treated with >2XIC50 drug for 2.5 hours |
Sample Preparation:
Sampleprep ID: | SP000469 |
Sampleprep Summary: | 90% Methanol/10% water at 50:1 solvent/cell pellet followed by vortexing and clarification via centrifugation |
Processing Method: | Lysis |
Processing Storage Conditions: | 4 C |
Extract Cleanup: | Clarification via centrifugation followed by nitrogen drying |
Extract Storage: | -80 C |
Sample Resuspension: | 6X pellet volume of HPLC H2O |
Sample Spiking: | Internal Standard 13C4, 15N1-Aspartate used for sample preparation normalizer |
Combined analysis:
Analysis ID | AN000692 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Thermo Exactive Orbitrap Plus |
Column | Phenomenex Synergi Hydro RP (100 x 2mm,2.5um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Plus Orbitrap |
Ion Mode | NEGATIVE |
Units | log2 fold change vs untreated |
Chromatography:
Chromatography ID: | CH000501 |
Chromatography Summary: | See Wenyun Lu et al Anal. Chem. 2010 or SA Cobbold et al JBC 2013 for detailed chromatography description |
Instrument Name: | Thermo Exactive Orbitrap Plus |
Column Name: | Phenomenex Synergi Hydro RP (100 x 2mm,2.5um) |
Internal Standard: | 13C4, 15N1-Aspartate |
Preconditioning: | Water Blanks |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS000615 |
Analysis ID: | AN000692 |
Instrument Name: | Thermo Q Exactive Plus Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
Ion Mode: | NEGATIVE |
Fragmentation Method: | None |
Mass Accuracy: | <1 ppm |