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MB Sample ID: SA022516
| Local Sample ID: | MMV666069 |
| Subject ID: | SU000462 |
| Subject Type: | MACS purified infected RBCs |
| Subject Species: | Plasmodium falciparum |
| Taxonomy ID: | 5833 |
| Species Group: | Microorganism |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000462 |
| Subject Type: | MACS purified infected RBCs |
| Subject Species: | Plasmodium falciparum |
| Taxonomy ID: | 5833 |
| Species Group: | Microorganism |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| MMV666069 | SA022516 | FL005521 | MMV666069 | Drug |
Collection:
| Collection ID: | CO000456 |
| Collection Summary: | Cells were pelleted and the media aspirated prior to methanol extraction |
| Sample Type: | Plasmodium cells |
Treatment:
| Treatment ID: | TR000476 |
| Treatment Summary: | Magnetically purified iRBCs (~20uL) were treated with >2XIC50 drug for 2.5 hours |
Sample Preparation:
| Sampleprep ID: | SP000469 |
| Sampleprep Summary: | 90% Methanol/10% water at 50:1 solvent/cell pellet followed by vortexing and clarification via centrifugation |
| Processing Method: | Lysis |
| Processing Storage Conditions: | 4 C |
| Extract Cleanup: | Clarification via centrifugation followed by nitrogen drying |
| Extract Storage: | -80 C |
| Sample Resuspension: | 6X pellet volume of HPLC H2O |
| Sample Spiking: | Internal Standard 13C4, 15N1-Aspartate used for sample preparation normalizer |
Combined analysis:
| Analysis ID | AN000692 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Exactive Orbitrap Plus |
| Column | Phenomenex Synergi Hydro RP (100 x 2mm,2.5um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Plus Orbitrap |
| Ion Mode | NEGATIVE |
| Units | log2 fold change vs untreated |
Chromatography:
| Chromatography ID: | CH000501 |
| Chromatography Summary: | See Wenyun Lu et al Anal. Chem. 2010 or SA Cobbold et al JBC 2013 for detailed chromatography description |
| Instrument Name: | Thermo Exactive Orbitrap Plus |
| Column Name: | Phenomenex Synergi Hydro RP (100 x 2mm,2.5um) |
| Internal Standard: | 13C4, 15N1-Aspartate |
| Preconditioning: | Water Blanks |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000615 |
| Analysis ID: | AN000692 |
| Instrument Name: | Thermo Q Exactive Plus Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Fragmentation Method: | None |
| Mass Accuracy: | <1 ppm |
