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MB Sample ID: SA022959
| Local Sample ID: | CE_G_AN_5_C |
| Subject ID: | SU000474 |
| Subject Type: | Bacterial Cells |
| Subject Species: | Staphylococcus aureus |
| Taxonomy ID: | 1280 |
| Species Group: | Microorganism |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000474 |
| Subject Type: | Bacterial Cells |
| Subject Species: | Staphylococcus aureus |
| Taxonomy ID: | 1280 |
| Species Group: | Microorganism |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| CE_G_AN_5_C | SA022959 | FL006418 | normal | Cell |
| CE_G_AN_5_C | SA022959 | FL006418 | Glucose | Additive 1 |
| CE_G_AN_5_C | SA022959 | FL006418 | none | Additive 2 |
| CE_G_AN_5_C | SA022959 | FL006418 | Anaerobic | Environment |
| CE_G_AN_5_C | SA022959 | FL006418 | not specified | NO status |
| CE_G_AN_5_C | SA022959 | FL006418 | not specified | Time |
Collection:
| Collection ID: | CO000468 |
| Collection Summary: | Cultures of S. aureus strain COL |
| Sample Type: | Bacterial cells |
Treatment:
| Treatment ID: | TR000488 |
| Treatment Summary: | Glucose NO 5 h AA NO 3 h Glucose Anaer Glucose NO 2 h Glucose Hypoxic Glucose Aerobic a.a. + Pyr Aer Glucose NO .25 h Glucose Anaerobic NO3 AA NO 1.5 h Glucose NO 3 h AA NO 2 h mut Gluc NO 1.5 h Glucose Anaerobic NO2 mut Gluc NO 3 h AA NO .5 h mut Gluc NO 4 h AA NO .25 h Glucose NO 1 h mut Gluc NO 1 h AA NO 4 h AA NO 2.5 h Glucose NO 2.5 h AA NO 1 h Glucose NO 1.5 h AA NO 2 h Glucose NO .5 h a.a. Aer Glucose + Pyruvate Aerobic |
Sample Preparation:
| Sampleprep ID: | SP000481 |
| Sampleprep Summary: | Methylillin-resistant S. aureus (MRSA) bacterial culture extracts re-suspended in water were provided to RTI RCMRC for analysis. These extracts were stored at -80 °C upon arrival. For processing, samples were dried overnight on a lyophilizer. The residue was then reconstituted with 150 µL of an ice-cold solution of reconstitution buffer (0.005 mg/mL Tryptophan-d5 in 95:5 Water:Methanol, v/v) and mixed on a multiple tube vortexer for 2 min at 5,000 rpm. Then, the samples were centrifuged at room temperature for 4 min at 16,000 rcf, and 100 µL of the supernatants were transferred to autosampler vials for data acquisition. Whole study pooled QC samples were created by combining 40 µL aliquot from each of the reconstituted samples into a 2 mL LoBind eppendorf tube. The QC pooled sample was then vortexed for 15 sec and 100 µL of the total study pooled QC samples were transferred to five pre-labeled autosampler vials. |
Combined analysis:
| Analysis ID | AN000709 | AN000710 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Waters Acquity | Waters Acquity |
| Column | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Waters Synapt G2 Si QTOF | Waters Synapt G2 Si QTOF |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | counts | counts |
Chromatography:
| Chromatography ID: | CH000513 |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) |
| Column Pressure: | 6,000-10,000 psi |
| Column Temperature: | 50 °C |
| Flow Rate: | 0.4 mL/min |
| Injection Temperature: | 8 °C |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% methanol; 0.1% formic acid |
| Analytical Time: | 22 min |
| Randomization Order: | Yes |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000631 |
| Analysis ID: | AN000709 |
| Instrument Name: | Waters Synapt G2 Si QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| MS ID: | MS000632 |
| Analysis ID: | AN000710 |
| Instrument Name: | Waters Synapt G2 Si QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
