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MB Sample ID: SA023087
Local Sample ID: | M_42 |
Subject ID: | SU000476 |
Subject Type: | Human Cell Media |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000476 |
Subject Type: | Human Cell Media |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Species Group: | Human |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
M_42 | SA023087 | FL005617 | Das. | Treatment |
M_42 | SA023087 | FL005617 | Unaffected | Growth_type |
Collection:
Collection ID: | CO000470 |
Collection Summary: | An aliquot of the media following treatment was collected, and the remainder of the media was aspirated. |
Sample Type: | Fibroblasts |
Treatment:
Treatment ID: | TR000490 |
Treatment Summary: | Primary cell cultures of desmoid tumor samples were established as previously described [1,2]. Monolayer cell cultures were grown in DMEM supplemented with 5% fetal bovine serum and maintained at 37°C in 5% CO2. Cells were divided when confluent and experiments were performed between the third and sixth passages. Approximately 10 x 106 cells were treated with 1.0uM Dasatinib (Selleck, Houston, USA) dissolved in DMSO, or 0.5uM FAK Inhibitor 14 (Cayman Chemicals Company, Michigan, USA) dissolved in water. Cells were incubated in fresh media containing the inhibitors, or vehicle, for 24 hours. |
Sample Preparation:
Sampleprep ID: | SP000483 |
Sampleprep Summary: | Cell media was extracted with 50% acetonitrile:water, vortexed, and centrifuged. An analytical quality control (QC) total pool was generated from equal aliquots from all of the media samples, divided into three total pool aliquots, and processed identically to the media study samples. All study and pool samples were lyophilized to dryness and reconstituted in a 0.2M phosphate buffer, pH 7.4, in D2O with 10% Chenomx ISTD and 6mM Imidazole. |
Analysis:
MB Sample ID: | SA023087 |
Analysis ID: | AN000712 |
Analysis Type: | NMR |
Num Factors: | 14 |
NMR:
NMR ID: | NM000079 |
Analysis ID: | AN000712 |
Instrument Name: | Bruker Avance III |
Instrument Type: | FT-NMR |
NMR Experiment Type: | 1D 1H |
Spectrometer Frequency: | 700 MHz |