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MB Sample ID: SA023291
Local Sample ID: | B_36 |
Subject ID: | SU000483 |
Subject Type: | Murine |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000483 |
Subject Type: | Murine |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Species Group: | Mammal |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
B_36 | SA023291 | FL005658 | 15 | Age |
B_36 | SA023291 | FL005658 | NTG | Genotype |
B_36 | SA023291 | FL005658 | F | Gender |
B_36 | SA023291 | FL005658 | CR | Diet |
Collection:
Collection ID: | CO000477 |
Collection Summary: | Brain (without hippocampus and entorhinal cortex) |
Sample Type: | Brain |
Treatment:
Treatment ID: | TR000497 |
Treatment Summary: | Calorie restricted (CR) and ad libitum (AL) diets |
Sample Preparation:
Sampleprep ID: | SP000490 |
Sampleprep Summary: | Brain tissue samples were homogenized in 50:50 Acetonitrile:Water , and the final concentration of all of the extracts was 1 mL/mg. Of the 71 samples, 48 had sufficient volume for study samples and for an analytical quality control (QC) total pool sample. This total pool was generated by transferring a 50 µL of each sample and aliquoting into six replicates, which were processed identically to the study samples. A 250 µL aliquot of each sample homogenate was lyophilized and then reconstituted with 250 µL of Phosphate buffer with Chenomx ISTD and 6mM Imidazole. Samples were vortexed and centrifuged before transferring 225 µL of each sample into pre-labeled 3mm NMR tubes for data acquisition on a 700 MHz spectrometer. |
Analysis:
MB Sample ID: | SA023291 |
Analysis ID: | AN000723 |
Analysis Type: | NMR |
Num Factors: | 17 |
NMR:
NMR ID: | NM000080 |
Analysis ID: | AN000723 |
Instrument Name: | Bruker Avance III |
Instrument Type: | FT-NMR |
NMR Experiment Type: | 1D 1H |
Spectrometer Frequency: | 700 MHz |