Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA023294

Local Sample ID:	B_68
Subject ID:	SU000483
Subject Type:	Murine
Subject Species:	Mus musculus
Taxonomy ID:	10090
Species Group:	Mammal

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Subject:

Subject ID:	SU000483
Subject Type:	Murine
Subject Species:	Mus musculus
Taxonomy ID:	10090
Species Group:	Mammal

Factors:

Local Sample ID	MB Sample ID	Factor Level ID	Level Value	Factor Name
B_68	SA023294	FL005659	15	Age
B_68	SA023294	FL005659	NTG	Genotype
B_68	SA023294	FL005659	M	Gender
B_68	SA023294	FL005659	AL	Diet

Collection:

Collection ID:	CO000477
Collection Summary:	Brain (without hippocampus and entorhinal cortex)
Sample Type:	Brain

Treatment:

Treatment ID:	TR000497
Treatment Summary:	Calorie restricted (CR) and ad libitum (AL) diets

Sample Preparation:

Sampleprep ID:	SP000490
Sampleprep Summary:	Brain tissue samples were homogenized in 50:50 Acetonitrile:Water , and the final concentration of all of the extracts was 1 mL/mg. Of the 71 samples, 48 had sufficient volume for study samples and for an analytical quality control (QC) total pool sample. This total pool was generated by transferring a 50 µL of each sample and aliquoting into six replicates, which were processed identically to the study samples. A 250 µL aliquot of each sample homogenate was lyophilized and then reconstituted with 250 µL of Phosphate buffer with Chenomx ISTD and 6mM Imidazole. Samples were vortexed and centrifuged before transferring 225 µL of each sample into pre-labeled 3mm NMR tubes for data acquisition on a 700 MHz spectrometer.

Sampleprep ID:

SP000490

Sampleprep Summary:

Brain tissue samples were homogenized in 50:50 Acetonitrile:Water , and the final concentration of all of the extracts was 1 mL/mg. Of the 71 samples, 48 had sufficient volume for study samples and for an analytical quality control (QC) total pool sample. This total pool was generated by transferring a 50 µL of each sample and aliquoting into six replicates, which were processed identically to the study samples. A 250 µL aliquot of each sample homogenate was lyophilized and then reconstituted with 250 µL of Phosphate buffer with Chenomx ISTD and 6mM Imidazole. Samples were vortexed and centrifuged before transferring 225 µL of each sample into pre-labeled 3mm NMR tubes for data acquisition on a 700 MHz spectrometer.

Analysis:

MB Sample ID:	SA023294
Analysis ID:	AN000723
Analysis Type:	NMR
Num Factors:	17

NMR:

NMR ID:	NM000080
Analysis ID:	AN000723
Instrument Name:	Bruker Avance III
Instrument Type:	FT-NMR
NMR Experiment Type:	1D 1H
Spectrometer Frequency:	700 MHz