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MB Sample ID: SA023370
Local Sample ID: | C_31 |
Subject ID: | SU000484 |
Subject Type: | Murine |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000484 |
Subject Type: | Murine |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Species Group: | Mammal |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
C_31 | SA023370 | FL005675 | 15 | Age |
C_31 | SA023370 | FL005675 | NTG | Genotype |
C_31 | SA023370 | FL005675 | F | Gender |
C_31 | SA023370 | FL005675 | CR | Diet |
Collection:
Collection ID: | CO000478 |
Collection Summary: | Cecal pellets |
Sample Type: | Brain |
Treatment:
Treatment ID: | TR000498 |
Treatment Summary: | Calorie restricted (CR) and ad libitum (AL) diets |
Sample Preparation:
Sampleprep ID: | SP000491 |
Sampleprep Summary: | Cecal samples were homogenized with HPLC grade water, and the final concentration of all of the extracts was 0.5 mg/µL. All 70 samples were included in an analytical quality control (QC) total pool. This total pool was created with 50 µL of each sample, which were divided into six replicates. A 250 µL aliquot of each sample and pool homogenate was filtered, and 225 µL of the filtrate was lyophilized. Lyophilized samples were reconstituted with 700 µL of Phosphate buffer with 0.5 mM DSS. Samples were vortexed and then centrifuged before transferring 600 µL of each sample into pre-labeled 5mm NMR tubes for data acquisition on a 700 MHz spectrometer. |
Analysis:
MB Sample ID: | SA023370 |
Analysis ID: | AN000724 |
Analysis Type: | NMR |
Num Factors: | 17 |
NMR:
NMR ID: | NM000081 |
Analysis ID: | AN000724 |
Instrument Name: | Bruker Avance III |
Instrument Type: | FT-NMR |
NMR Experiment Type: | 1D 1H |
Spectrometer Frequency: | 700 MHz |