Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA023394

Local Sample ID:	C_35
Subject ID:	SU000484
Subject Type:	Murine
Subject Species:	Mus musculus
Taxonomy ID:	10090
Species Group:	Mammal

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Subject:

Subject ID:	SU000484
Subject Type:	Murine
Subject Species:	Mus musculus
Taxonomy ID:	10090
Species Group:	Mammal

Factors:

Local Sample ID	MB Sample ID	Factor Level ID	Level Value	Factor Name
C_35	SA023394	FL005680	5	Age
C_35	SA023394	FL005680	APP	Genotype
C_35	SA023394	FL005680	M	Gender
C_35	SA023394	FL005680	AL	Diet

Collection:

Collection ID:	CO000478
Collection Summary:	Cecal pellets
Sample Type:	Brain

Treatment:

Treatment ID:	TR000498
Treatment Summary:	Calorie restricted (CR) and ad libitum (AL) diets

Sample Preparation:

Sampleprep ID:	SP000491
Sampleprep Summary:	Cecal samples were homogenized with HPLC grade water, and the final concentration of all of the extracts was 0.5 mg/µL. All 70 samples were included in an analytical quality control (QC) total pool. This total pool was created with 50 µL of each sample, which were divided into six replicates. A 250 µL aliquot of each sample and pool homogenate was filtered, and 225 µL of the filtrate was lyophilized. Lyophilized samples were reconstituted with 700 µL of Phosphate buffer with 0.5 mM DSS. Samples were vortexed and then centrifuged before transferring 600 µL of each sample into pre-labeled 5mm NMR tubes for data acquisition on a 700 MHz spectrometer.

Sampleprep ID:

SP000491

Sampleprep Summary:

Cecal samples were homogenized with HPLC grade water, and the final concentration of all of the extracts was 0.5 mg/µL. All 70 samples were included in an analytical quality control (QC) total pool. This total pool was created with 50 µL of each sample, which were divided into six replicates. A 250 µL aliquot of each sample and pool homogenate was filtered, and 225 µL of the filtrate was lyophilized. Lyophilized samples were reconstituted with 700 µL of Phosphate buffer with 0.5 mM DSS. Samples were vortexed and then centrifuged before transferring 600 µL of each sample into pre-labeled 5mm NMR tubes for data acquisition on a 700 MHz spectrometer.

Analysis:

MB Sample ID:	SA023394
Analysis ID:	AN000724
Analysis Type:	NMR
Num Factors:	17

NMR:

NMR ID:	NM000081
Analysis ID:	AN000724
Instrument Name:	Bruker Avance III
Instrument Type:	FT-NMR
NMR Experiment Type:	1D 1H
Spectrometer Frequency:	700 MHz