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MB Sample ID: SA023605
Local Sample ID: | TP_10 |
Subject ID: | SU000488 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000488 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Species Group: | Human |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
TP_10 | SA023605 | FL005705 | - | DM Type |
TP_10 | SA023605 | FL005705 | Total Pool_10 | A1C Category |
Collection:
Collection ID: | CO000482 |
Collection Summary: | Saliva samples were collected via standard operating procedures. |
Sample Type: | Saliva |
Treatment:
Treatment ID: | TR000502 |
Treatment Summary: | Saliva samples are from subjects with Type I and II diabetes. No treatment was given. |
Sample Preparation:
Sampleprep ID: | SP000495 |
Sampleprep Summary: | Study Samples and Whole Study Pooled QC Aliquots Preparation: Thawed aqueous saliva samples on ice. Samples were then vortexed and centrifuged at room temperature and at 16,000 rcf for four minutes. A 100 µL aliquot of each experimental sample was transferred to a labeled 2.0 mL Lo-Bind Eppendorf tube to make the study sample aliquots. Whole study pooled QC samples were created by combining 100 µL aliquot from each of the study samples into a 2 mL Lo-Bind eppendorf tube. This QC pooled sample was then vortexed for 30 seconds. Then, ten whole study pooled QC samples of 100 µL each were created. Sample Extraction To all samples (study and whole study pooled QC), 500 µL of Protein Precipitation Buffer with internal standard (0.0125 mg/mL of Tryptophan-d5 in methanol) was added to each tube and vortexed for 4 min at room temperature and at 5,000 rpm. The samples were then centrifuged at room temperature and at 16,000 rcf for 4 min. A 450 µL aliquot of the supernatant from each sample was transferred into pre-labeled 2.0 mL LoBind eppendorf tube and stored at -80 °C for one hour followed by a drying step on a lyophilizer. The residue was reconstituted in 100 µL of reconstitution buffer (95:5 Water:Methanol, v/v) and mixed on a multiple tube vortexer for 10 min at 5,000 rpm. Then, the samples were centrifuged at room temperature for 4 min at 16,000 rcf, and the supernatants were transferred to autosampler vials for data acquisition. |
Combined analysis:
Analysis ID | AN000730 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Waters Acquity |
Column | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) |
MS Type | ESI |
MS instrument type | QTOF |
MS instrument name | Waters |
Ion Mode | POSITIVE |
Units | m/z |
Chromatography:
Chromatography ID: | CH000523 |
Instrument Name: | Waters Acquity |
Column Name: | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS000647 |
Analysis ID: | AN000730 |
Instrument Name: | Waters |
Instrument Type: | QTOF |
MS Type: | ESI |
Ion Mode: | POSITIVE |