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MB Sample ID: SA023662
Local Sample ID: | OG1RF_3 Mup |
Subject ID: | SU000489 |
Subject Type: | Bacterial cells |
Subject Species: | Enterococcus faecalis |
Taxonomy ID: | 1351 |
Genotype Strain: | OG1RF |
Species Group: | Microorganism |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000489 |
Subject Type: | Bacterial cells |
Subject Species: | Enterococcus faecalis |
Taxonomy ID: | 1351 |
Genotype Strain: | OG1RF |
Species Group: | Microorganism |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
OG1RF_3 Mup | SA023662 | FL005720 | Mupirocin, 0.01mg/mL | Exposure |
Collection:
Collection ID: | CO000483 |
Collection Summary: | After the 15 minute treatment interval, 20mL sample will be harvested by filtration onto 0.45uM nitrocellulose membrane. The cells will be scraped off of the membrane into ice-cold PBS (~1mL), centrifuged for 45 seconds at 12K RPM, liquid aspirated and stored at -80C until sending for analysis. |
Sample Type: | Bacterial cells |
Collection Method: | Fast filtration |
Volumeoramount Collected: | 20ml |
Storage Conditions: | -80C |
Treatment:
Treatment ID: | TR000503 |
Treatment Summary: | Triplicate cultures of E. faecalis OG1RF will be grown in FMC-AUG to OD600 = 0.25. The cultures will be split into 3 equal volumes of 30mL which will be exposed to 100uL DMSO, 0.01mg/mL mupirocin, or 0.1mg/mL decoyinine for 15 minutes. At this point, 10mL sample will be removed for protein analysis. The remaining 20mL will be harvested by fast filtration for analysis.|Triplicate cultures of E. faecalis OG1RF will be grown in FMC-AUG to OD600 = 0.25. The cultures will be split into 3 equal volumes of 30mL which will be exposed to 100uL DMSO, 0.01mg/mL mupirocin, or 0.1mg/mL decoyinine for 15 minutes. At this point, 10mL sample will be removed for protein analysis. The remaining 20mL will be harvested by fast filtration for analysis.|Triplicate cultures of E. faecalis OG1RF will be grown in FMC-AUG to OD600 = 0.25. The cultures will be split into 3 equal volumes of 30mL which will be exposed to 100uL DMSO, 0.01mg/mL mupirocin, or 0.1mg/mL decoyinine for 15 minutes. At this point, 10mL sample will be removed for protein analysis. The remaining 20mL will be harvested by fast filtration for analysis. |
Treatment Compound: | DMSO|Mupirocin|Decoyinine |
Treatment Dose: | 100uL|0.01mg/mL|0.1mg/mL |
Treatment Doseduration: | 15 min|15 min|15 min |
Treatment Vehicle: | DMSO|DMSO|DMSO |
Sample Preparation:
Sampleprep ID: | SP000496 |
Sampleprep Summary: | NAD-ATP |
Sampleprep Protocol Filename: | SB_NAD-ATP_Assay_Agilent.pdf |
Combined analysis:
Analysis ID | AN000731 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Agilent 1290 Infinity |
Column | Thermo Hypercarb (50 x 2.1mm,3um) |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | Agilent 6490 QQQ |
Ion Mode | POSITIVE |
Units | nmol/mg protein |
Chromatography:
Chromatography ID: | CH000524 |
Chromatography Summary: | NAD-ATP |
Methods Filename: | SB_NAD-ATP_Assay_Agilent.pdf |
Instrument Name: | Agilent 1290 Infinity |
Column Name: | Thermo Hypercarb (50 x 2.1mm,3um) |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS000648 |
Analysis ID: | AN000731 |
Instrument Name: | Agilent 6490 QQQ |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
Ion Mode: | POSITIVE |
Analysis Protocol File: | SB_NAD-ATP_Assay_Agilent.pdf |