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MB Sample ID: SA024364
Local Sample ID: | S_18 |
Subject ID: | SU000497 |
Subject Type: | Murine |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000497 |
Subject Type: | Murine |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Species Group: | Mammal |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
S_18 | SA024364 | FL006166 | Veh | Treatment |
Collection:
Collection ID: | CO000491 |
Collection Summary: | Trunk blood was collected into centrifuge tubes and centrifuged for 15 min at ~1,000 x g at 4o C. Supernatant (serum) was extracted, frozen on dry ice, and stored at -80o C. |
Sample Type: | Blood |
Treatment:
Treatment ID: | TR000511 |
Treatment Summary: | BALB/cByJ male mice (4-5 weeks in age; Jackson Laboratory, Bar Harbor, ME) were given four treatments of either vehicle or OT (1.0 mg/kg; IP) across 8 days, with at least 48 hours between each injection. Mice were evaluated in a 3-chamber choice test for social preference 24 hr after the final injection. |
Sample Preparation:
Sampleprep ID: | SP000504 |
Sampleprep Summary: | 30 µL of each serum sample was transferred to a new, pre-labeled 1.5 mL LoBind eppendorf tube to make the individual study samples, and 12 µL was transferred to a new, pre-labeled 1.5 mL LoBind Eppendorf to make the total pool. The total pool was aliquoted into 3 Total Pool QC Samples and 4 Column Equilibrium samples with volumes of 30 µL in 1.5 mL LoBind Eppendorf tubes. 600 uL of Lipid Extraction Solvent (PC(17:0/17:0) 50 µg/mL in 2:1 dichloromethane:methanol) was added to all samples, and samples were vortexed at 4,000 rpm for 2mins on multiple-tube vortexer. 120 µL of H2O was added to each tube and the samples were vortexed at 4,000 rpm for 1min. Samples sat at room temperature for 10 minutes, and were centrifuged at 16,000 rcf for 10 min at 10°C. 240 µL of the lower lipid-rich DCM layer was transferred into new pre-labeled 1.5 mL LoBind Eppendorf tubes, and rubber stoppers were inserted. Samples were placed at -80˚C for 60 mins, and then lyophilized for 19.5 hrs. 240 µL of ACN/IPA/H2O (65:30:5 v/v/v) was added to each sample, and the samples were vortexed at 4,000 rpm for 2mins and centrifuged at 16,000 rcf for 4 min. 225 µL of the supernatant was transferred to pre-labeled autosampler vials and 10 µL was injected into OrbiTrap Velos. |
Combined analysis:
Analysis ID | AN000741 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Waters Acquity |
Column | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Orbitrap |
Ion Mode | POSITIVE |
Units | MS |
Chromatography:
Chromatography ID: | CH000533 |
Instrument Name: | Waters Acquity |
Column Name: | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS000658 |
Analysis ID: | AN000741 |
Instrument Name: | Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
Ion Mode: | POSITIVE |