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MB Sample ID: SA024407

Local Sample ID:S43_MS27
Subject ID:SU000498
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

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Subject:

Subject ID:SU000498
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
S43_MS27SA024407FL005892BLtime
S43_MS27SA024407FL005892controlgroup

Collection:

Collection ID:CO000492
Collection Summary:Fasting blood samples (5 ml) were collected from participants at baseline and the end of the 12 week intervention into vacutainers with EDTA
Sample Type:Blood

Treatment:

Treatment ID:TR000512
Treatment Summary:N/A

Sample Preparation:

Sampleprep ID:SP000505
Sampleprep Summary:The erythrocytes were separated from the plasma by centrifugation and frozen at -80ºC and were were prepared by hemolyzing the cells two times in deionized distilled water followed by centrifugation at 4000 RPM for 10 minutes at 4ºC. The erythrocyte aliquots were stored at −80 ºC until further analysis. The lipid extracts were prepared using a procedure by Rose and Oklander (Rose and Oklander 1965).

Combined analysis:

Analysis ID AN000742 AN000743
Analysis type MS MS
Chromatography type None (Direct infusion) None (Direct infusion)
Chromatography system None None
Column none none
MS Type ESI ESI
MS instrument type Triple quadrupole Triple quadrupole
MS instrument name Agilent 6460 QQQ Agilent 6460 QQQ
Ion Mode POSITIVE NEGATIVE
Units m/z value m/z value

Chromatography:

Chromatography ID:CH000534
Instrument Name:None
Column Name:none
Chromatography Type:None (Direct infusion)

MS:

MS ID:MS000659
Analysis ID:AN000742
Instrument Name:Agilent 6460 QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:POSITIVE
  
MS ID:MS000660
Analysis ID:AN000743
Instrument Name:Agilent 6460 QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:NEGATIVE
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