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MB Sample ID: SA025380
Local Sample ID: | B33 |
Subject ID: | SU000508 |
Subject Type: | Animal |
Subject Species: | Megalobrama amblycephala |
Taxonomy ID: | 75352 |
Species Group: | Fish |
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Subject:
Subject ID: | SU000508 |
Subject Type: | Animal |
Subject Species: | Megalobrama amblycephala |
Taxonomy ID: | 75352 |
Species Group: | Fish |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
B33 | SA025380 | FL005989 | High fat diet | Treatment |
Collection:
Collection ID: | CO000502 |
Collection Summary: | Blood samples were obtained following the standard protocol, using sodium heparin as anticoagulant. Liver tissue samples were weighed and put in tube then immediately flash-frozen with liquid nitrogen and stored at −80 °C till used for metabolomics analysis. |
Collection Protocol Filename: | Sample collection and NMR protocol.pdf |
Sample Type: | Plasma, Liver |
Treatment:
Treatment ID: | TR000522 |
Treatment Summary: | Three tanks were randomly assigned to each diet group (3 replicates x 4 diet-groups): control (C; containing 8.0% fat and 26.7% carbohydrates), high fat diet (HFD; containing 12.2% fat and 27.8% carbohydrates), high carbohydrate diet (HCD; containing 8.3% fat and 34.1% carbohydrates) and high fat high carbohydrate diet (HFHCD; containing 10.4% fat and 30.9% carbohydrates. |
Sample Preparation:
Sampleprep ID: | SP000515 |
Sampleprep Summary: | Each plasma sample (170 μL) was mixed with 340 μL phosphate buffer (45 mM, pH 7.47, 50% D2O) containing 0.9% NaCl in a 5 mm NMR tube and used directly for the NMR analysis. Liver tissues (about 50 mg) were homogenized in cold methanol and water (v/v=2:1) using a Qiagen Tissue-Lyser (Retsch GmBH, Germany). The supernatant (550uL) was collected after centrifugation (16 099 g, 4°C, 10 min). The extraction was repeated thrice using the same procedure. The supernatants collected from the above procedure were mixed together and freeze-dried after the removal of methanol in vacuo. Then the residue was dissolved in 600 μL of phosphate buffer (0.15 M, pH 7.38, 80% D2O) containing 0.001% TSP and 0.01% NaN3. The supernatant (550 μL) was transferred into a 5 mm NMR tube. |
Analysis:
MB Sample ID: | SA025380 |
Analysis ID: | AN000753 |
Analysis Type: | NMR |
Num Factors: | 4 |
NMR:
NMR ID: | NM000087 |
Analysis ID: | AN000753 |
Instrument Name: | Bruker AVance III |
Instrument Type: | FT-NMR |
NMR Experiment Type: | 1D 1H |
Field Frequency Lock: | Deuterium |
Standard Concentration: | 0.058mM |
Spectrometer Frequency: | 600 MHz |
NMR Probe: | cryo, inverse |
NMR Solvent: | D2O |
NMR Tube Size: | 5mm x 7 in |
Shimming Method: | topshim |