Return to study ST000494 main page
MB Sample ID: SA025610
| Local Sample ID: | ms5457-15 |
| Subject ID: | SU000515 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000515 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| ms5457-15 | SA025610 | FL006077 | treated-1 nM R1881 | Treatment |
| ms5457-15 | SA025610 | FL006077 | 72 hours | 13C media incubation time |
Collection:
| Collection ID: | CO000509 |
| Collection Summary: | LNCaP cell model |
| Sample Type: | Cultured cells |
Treatment:
| Treatment ID: | TR000529 |
| Treatment Summary: | LNCaP cells that were serum starved and incubated with 2mM [U-13C]glutamine and treated with dose response of a synthetic androgen (R1881) for 24 or 72 hours. |
Sample Preparation:
| Sampleprep ID: | SP000522 |
| Sampleprep Summary: | Add 50 uL 1x PBS solution to each vial containing cells. Cap, vortex, sonicate 2 x 20 sec bursts with vortexing inbetween and after Transfer entire contents of each vial into a labeled 1 dram vial. use 50 uL media for samples 9-16 use 50 uL plasma for QC Add 1.5 mL CHM solution to vial Add 20 uL 1:20 phenol red indicator Cap and vortex on multivortexer for 10 minutes Centrifuge @ 4C @ 3000 rpm for 10 minutes Aspirate and discard top phase hard to see the phase separation due to the light color (yellow). Dry bottom phase in speed vac Reconstitute samples and standards with 25 uL buffer A. (do triplicate injections) |
Combined analysis:
| Analysis ID | AN000760 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent 1290 Infinity |
| Column | Waters Acquity BEH C18 (150 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Agilent 6460 QQQ |
| Ion Mode | UNSPECIFIED |
| Units | 13C-palmitate % enrichment |
Chromatography:
| Chromatography ID: | CH000546 |
| Instrument Name: | Agilent 1290 Infinity |
| Column Name: | Waters Acquity BEH C18 (150 x 2.1mm,1.7um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000672 |
| Analysis ID: | AN000760 |
| Instrument Name: | Agilent 6460 QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| Ion Mode: | UNSPECIFIED |
