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MB Sample ID: SA025812
Local Sample ID: | iPSC_16 |
Subject ID: | SU000520 |
Subject Type: | Cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000520 |
Subject Type: | Cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Species Group: | Human |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
iPSC_16 | SA025812 | FL006029 | WT/L444P | Genotype |
Collection:
Collection ID: | CO000514 |
Collection Summary: | Cells were washed once with cold PBS, scraped off the plate in 1 ml cold PBS, transfered in pre-cooled 50 ml falcons and pelleted by centrifugation 3000rpm at 4°C. PBS was taken off and pellets were then snap frozen in liquid N2 and stored at -80°C. |
Sample Type: | Neurons |
Treatment:
Treatment ID: | TR000534 |
Treatment Summary: | 80% confluence, 48 hrs after last media change, differentiation medium (Neurobasal/B27/L-glut containing medium supplemented with BDNF, ascorbic acid , GDNF, TGFβ3, dibutyryl cAMP and DAPT. |
Sample Preparation:
Sampleprep ID: | SP000527 |
Sampleprep Summary: | none |
Sampleprep Protocol Filename: | SB_NAD-ATP_Assay_Agilent.pdf |
Combined analysis:
Analysis ID | AN000767 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Agilent 1290 Infinity |
Column | Thermo Hypercarb (50 x 2.1mm,3um) |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | Agilent 6490 QQQ |
Ion Mode | POSITIVE |
Units | nmol/mg protein |
Chromatography:
Chromatography ID: | CH000552 |
Methods Filename: | SB_NAD-ATP_Assay_Agilent.pdf |
Instrument Name: | Agilent 1290 Infinity |
Column Name: | Thermo Hypercarb (50 x 2.1mm,3um) |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS000679 |
Analysis ID: | AN000767 |
Instrument Name: | Agilent 6490 QQQ |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
Ion Mode: | POSITIVE |