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MB Sample ID: SA026040
Local Sample ID: | -8_36 |
Subject ID: | SU000524 |
Subject Type: | Humans |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Species Group: | Human |
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Subject:
Subject ID: | SU000524 |
Subject Type: | Humans |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Species Group: | Human |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
-8_36 | SA026040 | FL006564 | -8 | Time(weeks) |
-8_36 | SA026040 | FL006564 | GLP-1 analogue | Treatment |
Collection:
Collection ID: | CO000518 |
Collection Summary: | The samples analyzed were obtained in a fasting state. Samples were taken at inclusion, after 8 weeks of weight loss (baseline), and after 4 and 52 weeks of weight maintenance. |
Collection Protocol ID: | ClinicalTrials.gov Identifier: NCT02094183 |
Sample Type: | Blood |
Treatment:
Treatment ID: | TR000538 |
Treatment Summary: | 22 indivuals received 1.2 mg of liraglutide injections (Victoza, Novo Nordisk)daily for 1 year, 20 controls did not receive treatment |
Sample Preparation:
Sampleprep ID: | SP000531 |
Sampleprep Summary: | Prior to the NMR measurements, 260 μL of EDTA plasma and 260 μL of a sodium phosphate buffer (75 mmol/L Na2HPO4 in 80%/20% H2O/D2O, pH 7.4; including also 0.08% sodium 3-(trimethylsilyl)propionate-2,2,3,3-d4 and 0.04% sodium azide) was mixed and moved to the NMR tubes. All the liquid handling steps for plasma samples were done with a PerkinElmer JANUS Automated Workstation. The NMR metabolomics laboratory setup combined Bruker AVANCE III 500 MHz and Bruker AVANCE III HD 600 MHz spectrometers |
Analysis:
MB Sample ID: | SA026040 |
Analysis ID: | AN000771 |
Analysis Type: | NMR |
Num Factors: | 8 |
Num Metabolites: | 12 |
Units: | mmol/l |
NMR:
NMR ID: | NM000091 |
Analysis ID: | AN000771 |
Instrument Name: | Bruker Avance III 500 MHz and Bruker AVANCE III HD 600 MHz |
Instrument Type: | FT-NMR |
NMR Experiment Type: | 1D 1H |
Spectrometer Frequency: | 500 and 600 MHz |